WB (Western Blot)
(Detection of human JARID1C by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1C antibody AAA211284 (lot AAA211284-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human JARID1C Polyclonal Antibody | anti-JARID1C antibody
Rabbit anti-JARID1C Antibody, Affinity Purified
Gene Names
KDM5C; MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
Reactivity
Human
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JARID1C, Antibody; Rabbit anti-JARID1C Antibody, Affinity Purified; lysine-specific demethylase 5C; Smcy homolog, X-linked; Smcx homolog, X chromosome; SMCX; Protein Xe169; Protein SmcX; MRXSJ; MRXSCJ; XE169; DXS1272E; MRXJ; histone demethylase JARID1C; JARID1C; JmjC domain-containing protein SMCX; Jumonji, AT rich interactive domain 1C (RBP2-like); Jumonji/ARID domain-containing protein 1C; lysine (K)-specific demethylase 5C; MRX13; Lysine-specific demethylase 5C; lysine demethylase 5C; anti-JARID1C antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-JARID1C antibody
ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Dog, Pig
Immunogen
Between 275 and 325
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human JARID1C by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1C antibody AAA211284 (lot AAA211284-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human JARID1C by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, Jurkat, MCF-7, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JARID1C antibody AAA211284 (lot AAA211284-3) used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human JARID1C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1C antibody AAA211284 (lot AAA211284-3) used for IP at 6 ug per reaction. JARID1C was also immunoprecipitated by a previous lot of this antibody (lot AAA211284-2) and rabbit anti-JARID1C antibody For blotting immunoprecipitated JARID1C, AAA211284 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
IP (Immunoprecipitation)
(Detection of human JARID1C by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-JARID1C antibody AAA211284 (lot AAA211284-3) used for IP at 6 ug per reaction. JARID1C was also immunoprecipitated by a previous lot of this antibody (lot AAA211284-2) and rabbit anti-JARID1C antibody For blotting immunoprecipitated JARID1C, AAA211284 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.)
ChIP (Chromatin Immunoprecipitation)
(Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody AAA211284 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody AAA211284. ChIP-seq validation performed by Diagenode, Denville, NJ.)
ChIP (Chromatin Immunoprecipitation)
(Localization of Jarid1C Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-Jarid1C antibody AAA211284 and analyzed by DNA sequencing. The figure illustrates the peak distribution of Jarid1C binding within a 500 Kb region of chromosome 1 as detected using anti-Jarid1C antibody AAA211284. ChIP-seq validation performed by Diagenode, Denville, NJ.)
Related Product Information for anti-JARID1C antibody
JARID1C is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. Mutations in JARID1C are associated with X-linked mental retardation.
Product Categories/Family for anti-JARID1C antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
175,592 Da
NCBI Official Full Name
lysine-specific demethylase 5C isoform 1
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 5C
NCBI Official Symbol
KDM5C
NCBI Official Synonym Symbols
MRXJ; SMCX; MRX13; MRXSJ; XE169; MRXSCJ; JARID1C; DXS1272E
NCBI Protein Information
lysine-specific demethylase 5C; Smcy homolog, X-linked; Smcx homolog, X chromosome; histone demethylase JARID1C; JmjC domain-containing protein SMCX; Jumonji/ARID domain-containing protein 1C; Jumonji, AT rich interactive domain 1C (RBP2-like)
UniProt Protein Name
Lysine-specific demethylase 5C
UniProt Gene Name
KDM5C
UniProt Synonym Gene Names
DXS1272E; JARID1C; SMCX; XE169
UniProt Entry Name
KDM5C_HUMAN
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Product Notes
The JARID1C kdm5c (Catalog #AAA211284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JARID1C Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JARID1C can be used in a range of immunoassay formats including, but not limited to, ChIP (Chromatin immunoprecipitation), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JARID1C kdm5c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JARID1C, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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