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FCM/FACS (Flow Cytometry)
(JHDM1D Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human JHDM1D Polyclonal Antibody | anti-KDM7A antibody
JHDM1D Antibody (Center)
Gene Names
KDM7A; JHDM1D
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Synonyms
JHDM1D, Antibody; JHDM1D Antibody (Center); Lysine-specific demethylase 7A; 11411-; JmjC domain-containing histone demethylation protein 1D; Lysine-specific demethylase 7; KDM7A; JHDM1D; KDM7; KIAA1718; anti-KDM7A antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This JHDM1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-316 amino acids from the Central region of human JHDM1D.
Purity/Purification
This antibody is purified through a protein A column, followed by peptide affinity purification.
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Positions
290-316
Sequence Length
941
Applicable Applications for anti-KDM7A antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
Histone demethylase required for brain development. Specifically demethylates dimethylated 'Lys-9', 'Lys-27' and 'Lys-36' (H3K9me2, H3K27me2, H3K36me2, respectively) of histone H3 and monomethylated histone H4 'Lys-20' residue (H4K20Me1), thereby playing a central role in histone code (PubMed:20023638, PubMed:20622853). Specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. Demethylates H3K9me2 in absence of H3K4me3 (PubMed:20023638). Has activity toward H4K20Me1 only when nucleosome is used as a substrate and when not histone octamer is used as substrate (PubMed:20622853).
Cellular Location
Nucleus
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(JHDM1D Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(JHDM1D Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded H.testis tissue reacted with JHDM1D Antibody (Center))
IHC (Immunohistochemistry)
(Formalin-fixed and paraffin-embedded H.testis tissue reacted with JHDM1D Antibody (Center))
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded H.placenta tissue reacted with JHDM1D Antibody (Center))
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded H.placenta tissue reacted with JHDM1D Antibody (Center))
IHC (Immunohiostchemistry)
(JHDM1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JHDM1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(JHDM1D Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the JHDM1D Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of JHDM1D Antibody (Center) in 293 cell line lysates (35ug/lane).JHDM1D (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of JHDM1D Antibody (Center) in 293 cell line lysates (35ug/lane).JHDM1D (arrow) was detected using the purified Pab.)
Related Product Information for anti-KDM7A antibody
JHDM1D histone demethylase that specifically demethylates dimethylated 'Lys-9' and 'Lys-27' (H3K9me2 and H3K27me2, respectively) of histone H3, thereby playing a central role in histone code. This specifically binds trimethylated 'Lys-4' of histone H3 (H3K4me3), affecting histone demethylase specificity: in presence of H3K4me3, it has no demethylase activity toward H3K9me2, while it has high activity toward H3K27me2. This demethylates H3K9me2 in absence of H3K4me3.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
lysine-specific demethylase 7A
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 7A
NCBI Official Symbol
KDM7A
NCBI Official Synonym Symbols
JHDM1D
NCBI Protein Information
lysine-specific demethylase 7A
UniProt Protein Name
Lysine-specific demethylase 7A
UniProt Gene Name
KDM7A
UniProt Synonym Gene Names
JHDM1D; KDM7; KIAA1718
UniProt Entry Name
KDM7A_HUMAN
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Product Notes
The KDM7A kdm7a (Catalog #AAA284517) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 290-316. The JHDM1D Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JHDM1D can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KDM7A kdm7a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JHDM1D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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