Rabbit JHDM3A/JMJD2A/KDM4A Polyclonal Antibody | anti-KDM4A antibody
Anti-JHDM3A/JMJD2A/KDM4A Antibody Picoband
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
IHC-P: 2-5 ug/ml, Human, Mouse
FC/FACS: 1-3 ug/1x10^6 cells, Human
Direct ELISA: 0.1-0.5 ug/ml, Human
Tested Species: In-house tested species with positive results.
Enhanced Chemiluminescent Kit with anti-Rabbit IgG for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit for IHC(P).
FCM (Flow Cytometry)
(Figure 6. Flow Cytometry analysis of HepG2 cells using anti-JHDM3A/JMJD2A/KDM4A antibody (AAA19471).Overlay histogram showing HepG2 cells stained with AAA19471 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (AAA19471, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IHC (Immunohistochemistry)
(Figure 5. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (AAA19471).JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (AAA19471) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 4. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (AAA19471).JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human aryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (AAA19471) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 3. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (AAA19471).JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human squamous cell lung carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (AAA19471) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry)
(Figure 2. IHC analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (AAA19471).JHDM3A/JMJD2A/KDM4A was detected in a paraffin-embedded section of human ovarian serous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-JHDM3A/JMJD2A/KDM4A Antibody (AAA19471) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot)
(Figure 1. Western blot analysis of JHDM3A/JMJD2A/KDM4A using anti-JHDM3A/JMJD2A/KDM4A antibody (AAA19471).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human HEL whole cell lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JHDM3A/JMJD2A/KDM4A antigen affinity purified polyclonal antibody (#AAA19471) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for JHDM3A/JMJD2A/KDM4A at approximately 150 kDa. The expected band size for JHDM3A/JMJD2A/KDM4A is at 121 kDa.)
