WB (Western Blot)
(Detection of human and mouse JMJD2A by western blot. Samples: Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody AAA211187 (lot AAA211187-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse JMJD2A Polyclonal Antibody | anti-JMJD2A antibody
Rabbit anti-JMJD2A Antibody, Affinity Purified
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JMJD2A, Antibody; Rabbit anti-JMJD2A Antibody, Affinity Purified; jumonji domain containing 2; tudor domain containing 14A; TDRD14A; lysine-specific demethylase 4A; lysine (K)-specific demethylase 4A; jumonji domain containing 2A; jumonji C domain-containing histone demethylase 3A; JMJD2A; JMJD2; jmjC domain-containing histone demethylation protein 3A; JHDM3A; jumonji domain-containing protein 2A; Lysine-specific demethylase 4A; lysine demethylase 4A; anti-JMJD2A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-JMJD2A antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1014 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse JMJD2A by western blot. Samples: Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody AAA211187 (lot AAA211187-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse JMJD2A by western blot. Samples: Nuclear extract (50 ug) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-JMJD2A antibody AAA211187 (lot AAA211187-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-JMJD2A antibody AAA211187 (lot AAA211187-2) used for IP at 6 ug per reaction. JMJD2A was also immunoprecipitated by rabbit anti-JMJD2A antibody For blotting immunoprecipitated JMJD2A, AAA211187 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human JMJD2A by western blot of immunoprecipitates. Samples: Nuclear Extract (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-JMJD2A antibody AAA211187 (lot AAA211187-2) used for IP at 6 ug per reaction. JMJD2A was also immunoprecipitated by rabbit anti-JMJD2A antibody For blotting immunoprecipitated JMJD2A, AAA211187 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-JMJD2A antibody
JMJD2A is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. Some recently characterized members of the jmj family include JARID1A/RBP2, JARID1C, JMJD1A, JMJD1B, JMJD1C, JMJD2A, JMJD2C and JMJD2D. JMJD2A is a trimethylation-specific demethylase and functions as a transcriptional repressor. JMJD2A interacts with pRb and class one histone deacetylases to mediate repression of E2F-regulated promoters.
Product Categories/Family for anti-JMJD2A antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The JMJD2A kdm4a (Catalog #AAA211187) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JMJD2A Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JMJD2A kdm4a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD2A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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