Application Data
(HTRF assay for JMJD2A / KDM4A activity 1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2A / KDM4A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
JMJD2A/KDM4A recombinant protein
Recombinant JMJD2A/KDM4A protein
Gene Names
KDM4A; JMJD2; JHDM3A; JMJD2A; TDRD14A
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
JMJD2A/KDM4A; N/A; Recombinant JMJD2A/KDM4A protein; Lysine Demethylase 4A; Jumonji C Domain-Containing Histone Demethylase 3A; JMJD2A/KDM4A recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Full length recombinant JMJD2A/KDM4A protein was expressed in Sf9 cells and is supplied in 25mM Hepes pH7.5, 300mM NaCl, 10% Glycerol, 0.04% Triton X-100, 0.5mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
Application Data
(HTRF assay for JMJD2A / KDM4A activity 1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2A / KDM4A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for JMJD2A / KDM4A activity 1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2A / KDM4A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for JMJD2A / KDM4A activity 1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2A / KDM4A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Application Data
(HTRF assay for JMJD2A / KDM4A activity 1 uM H3K9me3 peptide was incubated with different concentrations of JMJD2A / KDM4A protein in a 10 ul reaction system containing 50 mM HEPES-NaOH pH 7.5, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2·6H2O for 1 hr, then 10 ul H3K9me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
SDS-PAGE
(Recombinant JMJD2A / KDM4A protein gel. JMJD2A / KDM4A protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 125.3 kDa Purity: > 85%)
SDS-PAGE
(Recombinant JMJD2A / KDM4A protein gel. JMJD2A / KDM4A protein was run on a 10% SDS-PAGE gel and stained with Coomassie blue. MW: 125.3 kDa Purity: > 85%)
Related Product Information for JMJD2A/KDM4A recombinant protein
Short Description: Recombinant JMJD2A/KDM4A (accession number NP_055478.2) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 125.3 kDa. KDM4A (lysine (K)-specific demethylase 4A), also known as JMJD2A (Jumonji Domain Containing 2A) is a nuclear protein that functions as a trimethylation-specific histone demethylase that preferentially demethylates trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to dimethylated form. KDM4A has no activity for mono- and dimethylated H3K9 and H3K36. KDM4A functions as a transcriptional repressor, participating in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. An additional KDM4A isoform, KDM4A Isoform 2, that lacks the N-terminal demethylase domain is crucial for muscle differentiation in promoting transcriptional activation of the MyoG gene by directing the removal of repressive chromatin marks at its promoter. Recombinant JMJD2A/KDM4A can be used for enzyme kinetics and inhibitor screening studies.
Background: KDM4A (lysine (K)-specific demethylase 4A), also known as JMJD2A (Jumonji Domain Containing 2A) is a nuclear protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to mono- or dimethylated form. JMJD2A/KDM4A has no activity for monomethylated H3K9 and H3K36. KDM4A functions as a transcriptional repressor, participating in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. An additional KDM4A isoform, KDM4A Isoform 2, that lacks the N-terminal demethylase domain is crucial for muscle differentiation in promoting transcriptional activation of the MyoG gene by directing the removal of repressive chromatin marks at its promoter.
Background: KDM4A (lysine (K)-specific demethylase 4A), also known as JMJD2A (Jumonji Domain Containing 2A) is a nuclear protein that functions as a histone demethylase that preferentially demethylates di- and trimethylated lysine 9 (K9me3) and lysine 36 (K36me3) residues of histone H3, converting these trimethylated histone residues to mono- or dimethylated form. JMJD2A/KDM4A has no activity for monomethylated H3K9 and H3K36. KDM4A functions as a transcriptional repressor, participating in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively. An additional KDM4A isoform, KDM4A Isoform 2, that lacks the N-terminal demethylase domain is crucial for muscle differentiation in promoting transcriptional activation of the MyoG gene by directing the removal of repressive chromatin marks at its promoter.
Product Categories/Family for JMJD2A/KDM4A recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
54,697 Da
NCBI Official Full Name
lysine-specific demethylase 4A
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4A
NCBI Official Symbol
KDM4A
NCBI Official Synonym Symbols
JMJD2; JHDM3A; JMJD2A; TDRD14A
NCBI Protein Information
lysine-specific demethylase 4A; jumonji domain containing 2; tudor domain containing 14A; jumonji domain containing 2A; jumonji domain-containing protein 2A; jumonji C domain-containing histone demethylase 3A; jmjC domain-containing histone demethylation
UniProt Protein Name
Lysine-specific demethylase 4A
UniProt Gene Name
KDM4A
UniProt Synonym Gene Names
JHDM3A; JMJD2; JMJD2A; KIAA0677
UniProt Entry Name
KDM4A_HUMAN
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Product Notes
The JMJD2A/KDM4A kdm4a (Catalog #AAA59694) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JMJD2A/KDM4A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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