WB (Western Blot)
(Detection of human JMJD2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody AAA211496 (lot AAA211496-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse JMJD2B Polyclonal Antibody | anti-JMJD2B antibody
Rabbit anti-JMJD2B Antibody, Affinity Purified
Gene Names
KDM4B; JMJD2B; TDRD14B
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
JMJD2B, Antibody; Rabbit anti-JMJD2B Antibody, Affinity Purified; jmjC domain-containing histone demethylation protein 3B; tudor domain containing 14B; TDRD14B; lysine-specific demethylase 4B; jumonji domain-containing protein 2B; JMJD2B; jumonji domain containing 2B; lysine (K)-specific demethylase 4B; Lysine-specific demethylase 4B; lysine demethylase 4B; anti-JMJD2B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-JMJD2B antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 1046 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human JMJD2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody AAA211496 (lot AAA211496-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human JMJD2B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody AAA211496 (lot AAA211496-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse JMJD2B by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody AAA211496 (lot AAA211496-2) used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of mouse JMJD2B by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody AAA211496 (lot AAA211496-2) used for WB at 0.5 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody AAA211496 (lot AAA211496-2) used for IP at 6 ug/mg lysate. JMJD2B was also immunoprecipitated by a previous lot (lot AAA211496-1) of this antibody For blotting immunoprecipitated JMJD2B, AAA211496-2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human JMJD2B by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-JMJD2B antibody AAA211496 (lot AAA211496-2) used for IP at 6 ug/mg lysate. JMJD2B was also immunoprecipitated by a previous lot (lot AAA211496-1) of this antibody For blotting immunoprecipitated JMJD2B, AAA211496-2 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-JMJD2B antibody
JMJD2B is a member of the jumonji (jmj) domain containing gene family of histone demethylases that plays a role in chromatin regulation and influences transcriptional activation and suppression. JMJD2B has been shown to be capable of antagonizing H3K9 trimethylation.
Product Categories/Family for anti-JMJD2B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121,897 Da
NCBI Official Full Name
lysine-specific demethylase 4B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4B
NCBI Official Symbol
KDM4B
NCBI Official Synonym Symbols
JMJD2B; TDRD14B
NCBI Protein Information
lysine-specific demethylase 4B; tudor domain containing 14B; jumonji domain containing 2B; jumonji domain-containing protein 2B; jmjC domain-containing histone demethylation protein 3B
UniProt Protein Name
Lysine-specific demethylase 4B
UniProt Gene Name
KDM4B
UniProt Synonym Gene Names
JHDM3B; JMJD2B; KIAA0876
UniProt Entry Name
KDM4B_HUMAN
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Product Notes
The JMJD2B kdm4b (Catalog #AAA211496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-JMJD2B Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the JMJD2B kdm4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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