FCM/FACS (Flow Cytometry)
(JMJD2B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human JMJD2B Polyclonal Antibody | anti-KDM4B antibody
JMJD2B Antibody (N-term)
Gene Names
KDM4B; JMJD2B; TDRD14B
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
JMJD2B, Antibody; JMJD2B Antibody (N-term); Lysine-specific demethylase 4B; 11411-; JmjC domain-containing histone demethylation protein 3B; Jumonji domain-containing protein 2B; KDM4B; JHDM3B; JMJD2B; KIAA0876; anti-KDM4B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This JMJD2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-31 amino acids from the N-terminal region of human JMJD2B.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
4-31
Sequence Length
448
Applicable Applications for anti-KDM4B antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(JMJD2B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(JMJD2B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human Testis tissue reacted with JMJD2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human Testis tissue reacted with JMJD2B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of JMJD2B Antibody (N-term) in K562 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of JMJD2B Antibody (N-term) in K562 cell line lysates (35ug/lane). JMJD2B (arrow) was detected using the purified Pab.)
Related Product Information for anti-KDM4B antibody
KDM4B / JMJD2B is a jumonji domain containing histone demethylase that specifically demethylates histone H3 at K9 and K36.
References
Beyer, S., et al. J. Biol. Chem. 283(52):36542-36552(2008)
Pollard, P.J., et al. Biochem. J. 416(3):387-394(2008)
Katoh, Y., et al. Int. J. Mol. Med. 20(2):269-273(2007)
Whetstine, J.R., et al. Cell 125(3):467-481(2006)
Gray, S.G., et al. J. Biol. Chem. 280(31):28507-28518(2005)
Pollard, P.J., et al. Biochem. J. 416(3):387-394(2008)
Katoh, Y., et al. Int. J. Mol. Med. 20(2):269-273(2007)
Whetstine, J.R., et al. Cell 125(3):467-481(2006)
Gray, S.G., et al. J. Biol. Chem. 280(31):28507-28518(2005)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
121897
NCBI Official Full Name
lysine-specific demethylase 4B
NCBI Official Synonym Full Names
lysine (K)-specific demethylase 4B
NCBI Official Symbol
KDM4B
NCBI Official Synonym Symbols
JMJD2B; TDRD14B
NCBI Protein Information
lysine-specific demethylase 4B
UniProt Protein Name
Lysine-specific demethylase 4B
UniProt Gene Name
KDM4B
UniProt Synonym Gene Names
JHDM3B; JMJD2B; KIAA0876
UniProt Entry Name
KDM4B_HUMAN
Similar Products
Product Notes
The KDM4B kdm4b (Catalog #AAA285058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 4-31. The JMJD2B Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JMJD2B can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KDM4B kdm4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JMJD2B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.