WB (Western Blot)
(Detection of human KARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-KARS antibody BL2472 (Cat. No. AAA211064) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). KARS was also immunoprecipitated by rabbit anti-KARS antibody BL2470. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
Rabbit anti-Human KARS Polyclonal Antibody | anti-KARS antibody
Rabbit anti-KARS Antibody, Affinity Purified
Gene Names
KARS; KRS; KARS1; KARS2; CMTRIB; DFNB89
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
KARS, Antibody; Rabbit anti-KARS Antibody, Affinity Purified; KARS1; lysRS; lysine--tRNA ligase; lysine tRNA ligase; KARS2; DFNB89; CMTRIB; Lysyl-tRNA synthetase; KRS; Lysine--tRNA ligase; lysyl-tRNA synthetase; anti-KARS antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-buffered Saline containing 0.1% BSA and 0.09% Sodium Azide
Concentration
200ug/ml (varies by lot)
Applicable Applications for anti-KARS antibody
ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 547 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human KARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-KARS antibody BL2472 (Cat. No. AAA211064) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). KARS was also immunoprecipitated by rabbit anti-KARS antibody BL2470. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
WB (Western Blot)
(Detection of human KARS by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-KARS antibody BL2472 (Cat. No. AAA211064) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate (B). KARS was also immunoprecipitated by rabbit anti-KARS antibody BL2470. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).)
IHC (Immunohistochemistry)
(Detection of human KARS by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS (Cat. No. AAA211064 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
IHC (Immunohistochemistry)
(Detection of human KARS by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-KARS (Cat. No. AAA211064 Lot1) used at a dilution of 1:200 (1ug/ml). Detection: DAB)
Related Product Information for anti-KARS antibody
KARS is an aminoacyl-tRNA synthetase that functions to charge tRNAs with their cognate amino acids.
Product Categories/Family for anti-KARS antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
71,497 Da
NCBI Official Full Name
lysine--tRNA ligase isoform 2
NCBI Official Synonym Full Names
lysyl-tRNA synthetase
NCBI Official Symbol
KARS
NCBI Official Synonym Symbols
KRS; KARS1; KARS2; CMTRIB; DFNB89
NCBI Protein Information
lysine--tRNA ligase; lysRS; lysine tRNA ligase
UniProt Protein Name
Lysine--tRNA ligase
UniProt Gene Name
KARS
UniProt Synonym Gene Names
KIAA0070; LysRS
UniProt Entry Name
SYK_HUMAN
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Product Notes
The KARS kars (Catalog #AAA211064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-KARS Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KARS can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IHC (Immunohistochemistry), IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KARS kars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KARS, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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