WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using Cytokeratin 5 Ab at 1/1000 dilution.Observed bands:65kD.)
Rabbit Keratin 5 Polyclonal Antibody | anti-KRT5 antibody
Keratin 5 Antibody
Gene Names
KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
Keratin 5, Antibody; Keratin 5 Antibody; 58 kDa cytokeratin; CK-5; CK5; Cytokeratin-5; Cytokeratin5; DDD; DDD1; EBS2; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; K2C5_HUMAN; K5; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin; keratin complex 2, basic, gene 5; keratin, type II cytoskeletal 5; Keratin-5; Keratin5; KRT 5; Krt5; KRT5A; type II cytoskeletal 5; Type-II keratin Kb5; anti-KRT5 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Keratin 5 antibody detects endogenous levels of total Keratin 5
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
-1mg/ml (varies by lot)
Sequence Length
590
Applicable Applications for anti-KRT5 antibody
ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human Cytokeratin 5, corresponding to a region within C-terminal amino acids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using Cytokeratin 5 Ab at 1/1000 dilution.Observed bands:65kD.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse testis, using Cytokeratin 5 Ab at 1/1000 dilution.Observed bands:65kD.)
Related Product Information for anti-KRT5 antibody
Description: K5 Belongs to the intermediate filament family. Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP.
Function: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Subunit Structure: Heterodimer of a type I and a type II keratin. Heterodimer with type I keratin KRT25 leading to the formation of keratin intermediate filament (KIF) network (PubMed:28899683). Heterodimer with KRT14 (PubMed:22705788). Interacts with TCHP (PubMed:15731013). Interacts with EPPK1 (By similarity).
Similarity: Belongs to the intermediate filament family.
Function: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
Subunit Structure: Heterodimer of a type I and a type II keratin. Heterodimer with type I keratin KRT25 leading to the formation of keratin intermediate filament (KIF) network (PubMed:28899683). Heterodimer with KRT14 (PubMed:22705788). Interacts with TCHP (PubMed:15731013). Interacts with EPPK1 (By similarity).
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62 kDa
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
KRT5
NCBI Official Synonym Symbols
K5; CK5; DDD; DDD1; EBS2; KRT5A
NCBI Protein Information
keratin, type II cytoskeletal 5
UniProt Protein Name
Keratin, type II cytoskeletal 5
UniProt Gene Name
KRT5
UniProt Synonym Gene Names
CK-5; K5
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Product Notes
The KRT5 krt5 (Catalog #AAA320839) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Keratin 5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 5 can be used in a range of immunoassay formats including, but not limited to, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KRT5 krt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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