Mouse anti-Human KRT5 Monoclonal Antibody | anti-KRT5 antibody

KRT5 antibody

Cat. Num. AAA48201

• Gene Names: KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: By protein-A affinity chromatography
• Synonyms: KRT5, Antibody; KRT5 antibody; Keratin 5; CK5; DDD; K5; KRT5A; anti-KRT5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a, k
• Clone Number: AT12C4
• Purity/Purification: By protein-A affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 590

• Applicable Applications for anti-KRT5 antibody: IHC (Immunohistochemistry), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), WB (Western Blot), ELISA
• Antigen Species: Human
• Immunogen: Recombinant human KRT5 (1-590aa) purified from E. coli
• Clone Note: Anti-human KRT5 mAb, clone AT12C4, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human KRT5 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

IF (Immunofluorescence)

(ICC/IF analysis of KRT5 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human KRT5 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

WB (Western Blot)

(The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human KRT5 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : HeLa cell lysate Lane 2. : A431 cell lysate)

Related Product Information for anti-KRT5 antibody

Keratin 5, also known as KRT5, K5, or CK5, dimerizes with keratin 14 and forms the intermediate filaments (IF) that make up the cytoskeleton of basal epithelial cells. This protein is involved in several diseases including epidermolysis bullosa simplex and breast and lung cancers. Keratin 5 (and K14) are expressed primarily in basal keratinocytes in the epidermis, specifically in the stratified epithelium lining the skin and digestive tract. Keratin intermediate filaments make up the cytoskeletal scaffold within epithelial cells, which contributes to the cell architecture and provides the cells with the ability to withstand mechanical, and non-mechanical, stresses. K5/K14 keratin pairs are able to undergo extensive bundling due to the non-helical tail of K15 acting as a weak cross-linker at the intermediate filament surface. This bundling increase the elasticity, and therefore the mechanical resilience, of the intermediate filaments.

Product Categories/Family for anti-KRT5 antibody

Signal Transduction

References

Lersch R., et al. (1988) Molecular and Cellular Biology. 8 (1): 486-493.; Eckert RL., et al. (1988) Dna. 7 (5): 337-345.; Atkinson SD., et al. (2011) J Invest Dermatol. 131 (10): 2079-2086.; van de Rijn M., et al. (2002) Am J Pathol. 161 (6): 1991-1996.

NCBI and Uniprot Product Information

• NCBI GI #: 119395754
• NCBI GeneID: 3852
• NCBI Accession #: NP_000415
• NCBI GenBank Nucleotide #: NM_000424.3
• UniProt Accession #: P13647; Q6PI71; Q6UBJ0; Q8TA91
• Molecular Weight: 62,378 Da
• NCBI Official Full Name: keratin, type II cytoskeletal 5
• NCBI Official Synonym Full Names: keratin 5
• NCBI Official Symbol: KRT5
• NCBI Official Synonym Symbols: K5; CK5; DDD; DDD1; EBS2; KRT5A
• NCBI Protein Information: keratin, type II cytoskeletal 5
• UniProt Protein Name: Keratin, type II cytoskeletal 5
• UniProt Gene Name: KRT5
• UniProt Synonym Gene Names: CK-5; K5