Rabbit anti-Human, Mouse KHDRBS1 Polyclonal Antibody | anti-KHDRBS1 antibody

KHDRBS1 Antibody (Center)

Cat. Num. AAA290628

• Gene Names: KHDRBS1; p62; p68; Sam68
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Synonyms: KHDRBS1, Antibody; KHDRBS1 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); KH domain-containing; RNA-binding; signal transduction-associated protein 1; GAP-associated tyrosine phosphoprotein p62; Src-associated in mitosis 68 kDa protein; Sam68; p21 Ras GTPase-activating protein-associated p62; p68; KHDRBS1 (HGNC:18116); anti-KHDRBS1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Sequence Length: 404

• Applicable Applications for anti-KHDRBS1 antibody: WB (Western Blot), IHC (Immunohistochemistry)
• Antigen Type: Synthesized Peptide
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(All lanes : Anti-KHDRBS1 Antibody (Center) at 1:2000 dilutionLane 1: Raji whole cell lysateLane 2: Hela whole cell lysateLane 3: Daudi whole cell lysateLane 4: HT-29 whole cell lysateLane 5: NIH/3T3 whole cell lysateLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

IHC (Immunohiostchemistry)

(AP21904c staining KHDRBS1 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

IHC (Immunohistochemistry)

(AP21904c staining KHDRBS1 in human colorectal carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37 degree C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Related Product Information for anti-KHDRBS1 antibody

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain- containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export. Positively regulates the association of constitutive transport element (CTE)-containing mRNA with large polyribosomes and translation initiation. According to some authors, is not involved in the nucleocytoplasmic export of unspliced (CTE)-containing RNA species according to (PubMed:22253824).

Product Categories/Family for anti-KHDRBS1 antibody

Crown Antibodies; Signal Transduction

References

Wong G.,et al.Cell 69:551-558(1992). Barlat I.,et al.J. Biol. Chem. 272:3129-3132(1997). Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 428673536
• NCBI GeneID: 10657
• NCBI Accession #: NP_001258807.1
• NCBI GenBank Nucleotide #: NM_001271878.1
• UniProt Accession #: Q07666; Q6PJX7; Q8NB97; Q99760; D3DPP3
• Molecular Weight: 44,027 Da
• NCBI Official Full Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1 isoform 2
• NCBI Official Synonym Full Names: KH domain containing, RNA binding, signal transduction associated 1
• NCBI Official Symbol: KHDRBS1
• NCBI Official Synonym Symbols: p62; p68; Sam68
• NCBI Protein Information: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• UniProt Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 1
• UniProt Gene Name: KHDRBS1
• UniProt Synonym Gene Names: Sam68
• UniProt Entry Name: KHDR1_HUMAN