WB (Western Blot)
(Western blot analysis of KHSRP using COLO205 whole cell lysates)
Rabbit KHSRP Polyclonal Antibody | anti-KHSRP antibody
KHSRP Antibody
Gene Names
KHSRP; p75; FBP2; KSRP; FUBP2
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KHSRP, Antibody; KHSRP Antibody; Far upstream element-binding protein 2; FBP2; FUBP2; FUBP2_HUMAN; FUSE binding protein 2; FUSE-binding protein 2; KH type splicing regulatory protein (FUSE binding protein 2); KH type splicing regulatory protein; KH type-splicing regulatory protein; KHSRP; KSRP; p75; anti-KHSRP antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KHSRP antibody detects endogenous levels of total KHSRP
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
711
Applicable Applications for anti-KHSRP antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot)
Immunogen
A synthesized peptide derived from human KHSRP
Subcellular Location
Nucleus. Cytoplasm. A small proportion is also found in the cytoplasm of neuronal cell bodies and dendrites.
Tissue Specificity
Detected in neural and non-neural cell lines.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Chicken, Xenopus
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Chicken (100%), Xenopus (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of KHSRP using COLO205 whole cell lysates)
WB (Western Blot)
(Western blot analysis of KHSRP using COLO205 whole cell lysates)
IHC (Immunohiostchemistry)
(AAA327986 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry)
(AAA327986 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence)
(AAA327986 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IF (Immunofluorescence)
(AAA327986 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Related Product Information for anti-KHSRP antibody
Function: Binds to the dendritic targeting element and may play a role in mRNA trafficking (By similarity). Part of a ternary complex that binds to the downstream control sequence (DCS) of the pre-mRNA. Mediates exon inclusion in transcripts that are subject to tissue-specific alternative splicing. May interact with single-stranded DNA from the far-upstream element (FUSE). May activate gene expression. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly by recruiting degradation machinery to ARE-containing mRNAs.
Subunit Structure: Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRPH1 (PubMed:11003644). Interacts with PARN (PubMed:15175153). Interacts with PQBP1 (PubMed:21933836).
Post-translational Modifications: Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.
Similarity: KH domains KH 3 and KH 4 behave as independent binding modules and can interact with different regions of the AU-rich RNA targets of degradation. Belongs to the KHSRP family.
Subunit Structure: Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRPH1 (PubMed:11003644). Interacts with PARN (PubMed:15175153). Interacts with PQBP1 (PubMed:21933836).
Post-translational Modifications: Phosphorylation at Ser-193 leads to the unfolding of the unstable KH domain 1, creating a site for 14-3-3 YWHAZ binding, which promotes nuclear localization and impairs the RNA degradation function.
Similarity: KH domains KH 3 and KH 4 behave as independent binding modules and can interact with different regions of the AU-rich RNA targets of degradation. Belongs to the KHSRP family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 73 kDa
Predicted: 74 kDa
Predicted: 74 kDa
NCBI Official Full Name
far upstream element-binding protein 2 isoform 1
NCBI Official Synonym Full Names
KH-type splicing regulatory protein
NCBI Official Symbol
KHSRP
NCBI Official Synonym Symbols
p75; FBP2; KSRP; FUBP2
NCBI Protein Information
far upstream element-binding protein 2
UniProt Protein Name
Far upstream element-binding protein 2
UniProt Gene Name
KHSRP
UniProt Synonym Gene Names
FUBP2; FUSE-binding protein 2; KSRP
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Product Notes
The KHSRP khsrp (Catalog #AAA327986) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KHSRP Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KHSRP can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot). Researchers should empirically determine the suitability of the KHSRP khsrp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KHSRP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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