IF (Immunofluorescence)
(Immunofluorescence staining of KHSRP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human KHSRP polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit anti-Human KHSRP Polyclonal Antibody | anti-KHSRP antibody
KHSRP Antibody, Rabbit PAb, Antigen Affinity Purified
Reactivity
Human
Applications
Immunofluorescence, Immunocytochemistry, Immunohistochemistry
Purity
Protein A & Antigen Affinity
Synonyms
KHSRP, Antibody; KHSRP Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-KHSRP Antibody, Rabbit Polyclonal; Anti-FBP2 Antibody; Anti-FUBP2 Antibody; Anti-KSRP Antibody; anti-KHSRP antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human KHSRP
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-KHSRP antibody
IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry)
Immunogen
E Coli-derived Human KHSRP fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human KHSRP fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2 degree C-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
IF (Immunofluorescence)
(Immunofluorescence staining of KHSRP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human KHSRP polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IF (Immunofluorescence)
(Immunofluorescence staining of KHSRP in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human KHSRP polyclonal antibody (dilution ratio 1:100) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human KHSRP in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohiostchemistry)
(Immunochemical staining of human KHSRP in human liver with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human KHSRP in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry)
(Immunochemical staining of human KHSRP in human brain with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)
Related Product Information for anti-KHSRP antibody
Background: KH-type splicing regulatory protein (KHSRP) is a single-stranded nucleic acid binding protein with roles in transcription and mRNA decay, and it is also a component of the Drosha-DGCR8 complex promoting the miRNA biogenesis. KHSRP modulates RNA life and gene expression at various levels. KHSRP controls important cellular functions as different as proliferation, differentiation, metabolism, and response to infectious agents. KHSRP plays an important role in small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC) tumorigenesis and could be a potential prognostic marker and novel therapeutic target for SCLC and NSCLC treatment.
References
Yuan H, et al. (2017) Sumo1 modification of khsrp regulates tumorigenesis by preventing the tl-g-rich mirna biogenesis. Mol Cancer 16 (1): 157.
Yan M, et al. (2019) Rna-binding protein khsrp promotes tumor growth and metastasis in non-small cell lung cancer. J Exp Clin Cancer Res 38 (1): 478
.Briata P, et al. (2016) Diverse roles of the nucleic acid-binding protein khsrp in cell differentiation and disease. Wiley Interdiscip Rev RNA 7 (2): 227-240.
Yan M, et al. (2019) Rna-binding protein khsrp promotes tumor growth and metastasis in non-small cell lung cancer. J Exp Clin Cancer Res 38 (1): 478
.Briata P, et al. (2016) Diverse roles of the nucleic acid-binding protein khsrp in cell differentiation and disease. Wiley Interdiscip Rev RNA 7 (2): 227-240.
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Product Notes
The KHSRP (Catalog #AAA258285) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KHSRP Antibody, Rabbit PAb, Antigen Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KHSRP can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ICC (Immunocytochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the KHSRP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KHSRP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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