WB (Western Blot)
(Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67, AAA76741, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.)
Rabbit anti-Mouse, Rat Ki67 Polyclonal Antibody | anti-Ki67 antibody
Ki67
Gene Names
MKI67; KIA
Reactivity
Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot, Immunocytochemistry
Synonyms
Ki67, Antibody; Ki67; anti-Ki67 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Liquid. Supplied as an aliquot of serum plus 5mM sodium azide
Applicable Applications for anti-Ki67 antibody
IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Recombinant segment of mouse sequence expressed in and purified from E coli.
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67, AAA76741, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.)
WB (Western Blot)
(Western blot analysis of equal amounts of cell lysates using rabbit pAb to Ki67, AAA76741, dilution 1:10,000, (green): [1] protein standard (red), [2] rapidly growing HeLa cell cultures, [3] rapidly growing HEK293 cell cultures. Strong double bands larger than the 250kDa standard correspond to full length 345kDa and 395kDa Ki67 isoforms, while smaller proteolytic fragments of these isoforms are also invariably detected on the blot.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67, AAA76741, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.)
IF (Immunofluorescence)
(Immunofluorescent analysis of HeLa cells stained with rabbit pAb to Ki67, AAA76741, dilution 1:5,000 in red, and mouse monoclonal antibody to fibrillarin in green. The blue is DAPI staining of nuclear DNA. The Ki67 protein accumulates in and around the nucleoli of interphase cells such as those on the right, and the nucleoli are revealed by the fibrillarin antibody. In contrast, cells in the quiescent G0 state such as those on the left are Ki67 negative but fibrillarin positive.)
Related Product Information for anti-Ki67 antibody
The Ki67 protein was first discovered when researchers attempted to generate cancer cell specific monoclonal antibodies by injecting mice with nuclear preparations from Hodgkin's lymphoma cells. The presence of the Ki67 protein is frequently used as an indicator of cell proliferation and its level of expression is one of the most reliable biomarkers of proliferative status of cancer cells. Much research shows a correlation between Ki67 protein level and prognosis in cancer patients, when high Ki67 levels being associated with poorer outcomes.
It was made against a recombinant construct including amino the acids 956-1,322 of the mouse sequence XP_006507475.1, a region corresponding to 2nd, 3rd and 4th Ki67 type repeats. Since the Ki67 protein is relatively poorly conserved in amino acid sequence, this antibody is not recommended for use on human tissues, for which RA22150 antibody, made against the human protein, would be superior. Note that the Ki67 proteins are very unstable and only expressed in large amounts in situations where many cells are dividing. As a result of the very short half life of Ki67 there are usually numerous fragments visible on western blots running below the major 395kDa and 345kDa bands.
It was made against a recombinant construct including amino the acids 956-1,322 of the mouse sequence XP_006507475.1, a region corresponding to 2nd, 3rd and 4th Ki67 type repeats. Since the Ki67 protein is relatively poorly conserved in amino acid sequence, this antibody is not recommended for use on human tissues, for which RA22150 antibody, made against the human protein, would be superior. Note that the Ki67 proteins are very unstable and only expressed in large amounts in situations where many cells are dividing. As a result of the very short half life of Ki67 there are usually numerous fragments visible on western blots running below the major 395kDa and 345kDa bands.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358,694 Da
NCBI Official Full Name
antigen KI-67 isoform 2
NCBI Official Synonym Full Names
antigen identified by monoclonal antibody Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA
NCBI Protein Information
antigen KI-67; proliferation-related Ki-67 antigen
UniProt Protein Name
Antigen KI-67
UniProt Gene Name
MKI67
UniProt Entry Name
KI67_HUMAN
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Product Notes
The Ki67 mki67 (Catalog #AAA76741) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ki67 reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ki67 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the Ki67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki67, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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