WB (Western Blot)
(Detection of human and mouse KIF5B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-KIF5B antibody AAA212608 (lot AAA212608-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Rabbit anti-Human, Mouse KIF5B Polyclonal Antibody | anti-KIF5B antibody
Rabbit anti-KIF5B Antibody, Affinity Purified
Gene Names
KIF5B; KNS; KINH; KNS1; UKHC; HEL-S-61
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
KIF5B, Antibody; Rabbit anti-KIF5B Antibody, Affinity Purified; kinesin 1 (110-120kD); UKHC; ubiquitous kinesin heavy chain; KNS1; KNS; KINH; kinesin heavy chain; HEL-S-61; epididymis secretory protein Li 61; conventional kinesin heavy chain; kinesin-1 heavy chain; Kinesin-1 heavy chain; kinesin family member 5B; anti-KIF5B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-KIF5B antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 675 and 725
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse KIF5B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-KIF5B antibody AAA212608 (lot AAA212608-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
WB (Western Blot)
(Detection of human and mouse KIF5B by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-KIF5B antibody AAA212608 (lot AAA212608-1) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human KIF5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells. Antibodies: Affinity purified rabbit anti-KIF5B antibody AAA212608 (lot AAA212608-1) used for IP at 6 ug per reaction. KIF5B was also immunoprecipitated by rabbit anti-KIF5B antibody For blotting immunoprecipitated KIF5B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
IP (Immunoprecipitation)
(Detection of human KIF5B by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T and cells. Antibodies: Affinity purified rabbit anti-KIF5B antibody AAA212608 (lot AAA212608-1) used for IP at 6 ug per reaction. KIF5B was also immunoprecipitated by rabbit anti-KIF5B antibody For blotting immunoprecipitated KIF5B, was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 1 second.)
Related Product Information for anti-KIF5B antibody
Kinesin family member 5B (KIF5B) is a microtubule-dependent motor required for normal distribution of mitochondria and lysosomes (By similarity) [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/P33176].
Product Categories/Family for anti-KIF5B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
109,685 Da
NCBI Official Full Name
kinesin-1 heavy chain
NCBI Official Synonym Full Names
kinesin family member 5B
NCBI Official Symbol
KIF5B
NCBI Official Synonym Symbols
KNS; KINH; KNS1; UKHC; HEL-S-61
NCBI Protein Information
kinesin-1 heavy chain
UniProt Protein Name
Kinesin-1 heavy chain
UniProt Gene Name
KIF5B
UniProt Synonym Gene Names
KNS; KNS1; UKHC
UniProt Entry Name
KINH_HUMAN
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Product Notes
The KIF5B kif5b (Catalog #AAA212608) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-KIF5B Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIF5B can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the KIF5B kif5b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF5B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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