Rat Kinesin Family, Member 5B (KIF5B) ELISA Kit | KIF5B elisa kit

Rat Kinesin Family, Member 5B (KIF5B) ELISA Kit

Cat. Num. AAA186856

• Gene Names: KIF5B; KNS; KINH; KNS1; UKHC; HEL-S-61
• Reactivity: Rat
• Synonyms: Kinesin Family, Member 5B (KIF5B); N/A; Rat Kinesin Family, Member 5B (KIF5B) ELISA Kit; KNS1; UKHC; KINH; Conventional kinesin heavy chain; Ubiquitous kinesin heavy chain; Kinesin-1 heavy chain; KIF5B elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Kinesin Family, Member 5B (KIF5B).; No significant cross-reactivity or interference between Kinesin Family, Member 5B (KIF5B) and analogues was observed.
• Sequence Length: 963
• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL

• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kinesin Family, Member 5B (KIF5B) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kinesin Family, Member 5B (KIF5B) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Standard Curve (Sample)

Related Product Information for KIF5B elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 5B (KIF5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Family, Member 5B (KIF5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 5B (KIF5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Kinesin Family, Member 5B (KIF5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119606383
• NCBI GeneID: 3799
• UniProt Accession #: P33176; Q5VZ85; A0AVB2
• Molecular Weight: 109,685 Da
• NCBI Official Full Name: kinesin family member 5B, isoform CRA_a
• NCBI Official Synonym Full Names: kinesin family member 5B
• NCBI Official Symbol: KIF5B
• NCBI Official Synonym Symbols: KNS; KINH; KNS1; UKHC; HEL-S-61
• NCBI Protein Information: kinesin-1 heavy chain
• UniProt Protein Name: Kinesin-1 heavy chain
• UniProt Gene Name: KIF5B
• UniProt Synonym Gene Names: KNS; KNS1; UKHC