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Application Data
(Fluorescent confocal image of U251 cellstained with KLF9 Antibody(N-term).U251 cells were fixedwith 4% PFA (20 min), permeabilized withTriton X-100 (0.1%, 10 min), then incubatedwith KLF9 primary antibody (1:25, 1 h at 37?).For secondary antibody, Alexa Fluor® 488conjugated donkey anti-rabbit antibody (green)was used (1:400, 50 min at 37?).Cytoplasmicactin was counterstained with Alexa Fluor®555 (red) conjugated Phalloidin (7units/ml, 1 hat 37?). Nuclei were counterstained with DAPI(blue) (10 ug/ml, 10 min). KLF9immunoreactivity is localized to vesicles andNucleus significantly.)
Rabbit KLF9 Polyclonal Antibody | anti-KLF9 antibody
KLF9 Antibody (N-term)
Gene Names
KLF9; BTEB; BTEB1
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
KLF9, Antibody; KLF9 Antibody (N-term); Krueppel-like factor 9; Basic transcription element-binding protein 1; BTE-binding protein 1; GC-box-binding protein 1; Transcription factor BTEB1; KLF9; BTEB; BTEB1; anti-KLF9 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This KLF9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of human KLF9.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
29-57
Applicable Applications for anti-KLF9 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Cellular location
nucleus
Tissue location
Epidermis (at protein level)
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Application Data
(Fluorescent confocal image of U251 cellstained with KLF9 Antibody(N-term).U251 cells were fixedwith 4% PFA (20 min), permeabilized withTriton X-100 (0.1%, 10 min), then incubatedwith KLF9 primary antibody (1:25, 1 h at 37?).For secondary antibody, Alexa Fluor® 488conjugated donkey anti-rabbit antibody (green)was used (1:400, 50 min at 37?).Cytoplasmicactin was counterstained with Alexa Fluor®555 (red) conjugated Phalloidin (7units/ml, 1 hat 37?). Nuclei were counterstained with DAPI(blue) (10 ug/ml, 10 min). KLF9immunoreactivity is localized to vesicles andNucleus significantly.)
Application Data
(Fluorescent confocal image of U251 cellstained with KLF9 Antibody(N-term).U251 cells were fixedwith 4% PFA (20 min), permeabilized withTriton X-100 (0.1%, 10 min), then incubatedwith KLF9 primary antibody (1:25, 1 h at 37?).For secondary antibody, Alexa Fluor® 488conjugated donkey anti-rabbit antibody (green)was used (1:400, 50 min at 37?).Cytoplasmicactin was counterstained with Alexa Fluor®555 (red) conjugated Phalloidin (7units/ml, 1 hat 37?). Nuclei were counterstained with DAPI(blue) (10 ug/ml, 10 min). KLF9immunoreactivity is localized to vesicles andNucleus significantly.)
Application Data
(KLF9 Antibody (N-term)western blot analysis in WiDr cell line lysates(35ug/lane).This demonstrates the KLF9antibody detected the KLF9 protein (arrow).)
Application Data
(KLF9 Antibody (N-term)western blot analysis in WiDr cell line lysates(35ug/lane).This demonstrates the KLF9antibody detected the KLF9 protein (arrow).)
Application Data
(Anti-KLF9 Antibody (N-term) at 1:2000 dilution+ U-87 MG whole cell lysate Lysates/proteins at20 ug per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(Anti-KLF9 Antibody (N-term) at 1:2000 dilution+ U-87 MG whole cell lysate Lysates/proteins at20 ug per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000dilution. Predicted band size : 27 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(All lanes : Anti-KLF9 Antibody (N-term) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Application Data
(All lanes : Anti-KLF9 Antibody (N-term) at 1:1000 dilution Lane 1: C6 whole cell lysate Lane 2: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 27 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-KLF9 antibody
The protein encoded by this gene is a transcription factor
that binds to GC box elements located in the promoter. Binding of
the encoded protein to a single GC box inhibits mRNA expression
while binding to tandemly repeated GC box elements activates
transcription.
that binds to GC box elements located in the promoter. Binding of
the encoded protein to a single GC box inhibits mRNA expression
while binding to tandemly repeated GC box elements activates
transcription.
References
Du, H., et al. Biol. Reprod. 83(2):205-211(2010)
Pabona, J.M., et al. Endocrinology 151(7):3396-3406(2010)
Gamper, I., et al. Exp. Hematol. 37(5):539-548(2009)
Kang, L., et al. Pathol. Int. 58(6):334-338(2008)
Simmen, F.A., et al. Reprod. Biol. Endocrinol. 6, 41 (2008) :
Pabona, J.M., et al. Endocrinology 151(7):3396-3406(2010)
Gamper, I., et al. Exp. Hematol. 37(5):539-548(2009)
Kang, L., et al. Pathol. Int. 58(6):334-338(2008)
Simmen, F.A., et al. Reprod. Biol. Endocrinol. 6, 41 (2008) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Krueppel-like factor 9
NCBI Official Synonym Full Names
Kruppel-like factor 9
NCBI Official Symbol
KLF9
NCBI Official Synonym Symbols
BTEB; BTEB1
NCBI Protein Information
Krueppel-like factor 9
UniProt Protein Name
Krueppel-like factor 9
UniProt Gene Name
KLF9
UniProt Synonym Gene Names
BTEB; BTEB1; BTE-binding protein 1
UniProt Entry Name
KLF9_HUMAN
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Product Notes
The KLF9 klf9 (Catalog #AAA289197) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 29-57. The KLF9 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Pig, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's KLF9 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the KLF9 klf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLF9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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