IF (Immunofluorescence)
(AAA329102 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329102) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Rabbit KMT5C/SUV4-20h2 Polyclonal Antibody | anti-KMT5C antibody
KMT5C/SUV4-20h2 Antibody
Gene Names
KMT5C; SUV420H2; Suv4-20h2
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
KMT5C/SUV4-20h2, Antibody; KMT5C/SUV4-20h2 Antibody; FLJ98627; Histone lysine N methyltransferase SUV420H2; Histone-lysine N-methyltransferase SUV420H2; KMT5C; Lysine N methyltransferase 5C; Lysine N-methyltransferase 5C; MGC2705; Su(var)4 20 homolog 2; Su(var)4-20 homolog 2; Suppressor of variegation 4 20 homolog 2; Suppressor of variegation 4-20 homolog 2; Suv4 20h2; Suv4-20h2; SUV420H2; SV422_HUMAN; anti-KMT5C antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)
Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%)
Clonality
Polyclonal
Isotype
IgG
Specificity
KMT5C/SUV4-20h2 Antibody detects endogenous levels of total KMT5C/SUV4-20h2.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
462
Applicable Applications for anti-KMT5C antibody
ELISA
Immunogen
A synthesized peptide derived from human KMT5C/SUV4-20h2, corresponding to a region within the internal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
IF (Immunofluorescence)
(AAA329102 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329102) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
IF (Immunofluorescence)
(AAA329102 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA329102) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
WB (Western Blot)
(Western blot analysis of extracts from Mouse stomach, using KMT5C/SUV4-20h2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Mouse stomach, using KMT5C/SUV4-20h2 Antibody. The lane on the left was treated with blocking peptide.)
WB (Western Blot)
(Western blot analysis of extracts from Hybridoma cells, using KMT5C/SUV4-20h2 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 60 kDa.)
WB (Western Blot)
(Western blot analysis of extracts from Hybridoma cells, using KMT5C/SUV4-20h2 Antibody. The lane on the left was treated with blocking peptide.Observed bands: 60 kDa.)
Related Product Information for anti-KMT5C antibody
Function: Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity (PubMed:24396869, PubMed:28114273). In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes (PubMed:24396869). H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5C is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (PubMed:28114273). May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
Subcellular Location: Nucleus. Chromosome. Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).
Subunit Structure: Homodimer (By similarity). Interacts with HP1 proteins CBX1, CBX3 and CBX5. Interacts with RB1 family proteins RB1, RBL1 and RBL2 (By similarity).
Similarity: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
Subcellular Location: Nucleus. Chromosome. Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).
Subunit Structure: Homodimer (By similarity). Interacts with HP1 proteins CBX1, CBX3 and CBX5. Interacts with RB1 family proteins RB1, RBL1 and RBL2 (By similarity).
Similarity: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)52kD, 60kD.
Predicted Molecular Weight: (Calculated)52kDa.
Predicted Molecular Weight: (Calculated)52kDa.
NCBI Official Full Name
Histone-lysine N-methyltransferase KMT5C
NCBI Official Synonym Full Names
lysine methyltransferase 5C
NCBI Official Symbol
KMT5C
NCBI Official Synonym Symbols
SUV420H2; Suv4-20h2
NCBI Protein Information
histone-lysine N-methyltransferase KMT5C
UniProt Protein Name
Histone-lysine N-methyltransferase SUV420H2
UniProt Gene Name
SUV420H2
UniProt Synonym Gene Names
KMT5C
UniProt Entry Name
SV422_HUMAN
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Product Notes
The KMT5C suv420h2 (Catalog #AAA329102) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KMT5C/SUV4-20h2 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Zebrafish(88%), Bovine(100%), Horse(88%), Sheep(100%), Dog(100%) and may cross-react with other species as described in the data sheet. AAA Biotech's KMT5C/SUV4-20h2 can be used in a range of immunoassay formats including, but not limited to, ELISA. Researchers should empirically determine the suitability of the KMT5C suv420h2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KMT5C/SUV4-20h2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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