Rabbit anti-Human, Mouse KPNA2 Polyclonal Antibody | anti-KPNA2 antibody

KPNA2 Antibody

Cat. Num. AAA232557

• Gene Names: KPNA2; QIP2; RCH1; IPOA1; SRP1alpha; SRP1-alpha
• Reactivity: Human, Mouse
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot, ELISA
• Purity: >95%, Protein G purified
• Synonyms: KPNA2, Antibody; KPNA2 Antibody; Importin subunit alpha-1; Karyopherin subunit alpha-2; RAG cohort protein 1; SRP1-alpha; KPNA2; RCH1; SRP1; anti-KPNA2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 529

• Applicable Applications for anti-KPNA2 antibody: IF (Immunofluorescence), IHC (Immunohistochemistry), WB (Western Blot), ELISA
• Immunogen: Recombinant human Importin subunit alpha-1 protein (2-285AA)
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Conjugation: Non-conjugated
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6917 / sc-25522
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

WB (Western Blot)

(Western BlotPositive WB detected in:HepG2 whole cell lysate,Raji whole cell lysate,Jurkat whole cell lysate,HEK293 whole cell lysate,NIH/3T3 whole cell lysate,A549 whole cell lysate,U251 whole cell lysateAll lanes: KPNA2 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 58 kDaObserved band size: 58,36 kDa)

IHC (Immunohistochemisry)

(Immunofluorescent analysis of HepG2 cells using AAA232557 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

IHC (Immunohiostchemistry)

(Immunohistochemistry of paraffin-embedded human colonic tissue using AAA232557 at dilution of 1:100)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human lymph node tissue using AAA232557 at dilution of 1:100)

Related Product Information for anti-KPNA2 antibody

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

NCBI and Uniprot Product Information

• NCBI GI #: 1002341781
• NCBI GeneID: 3838
• NCBI Accession #: NP_001307540.1
• NCBI GenBank Nucleotide #: NM_001320611.1
• UniProt Accession #: P52292; Q53YE3; Q9BRU5; B9EJD6
• Molecular Weight: 57,862 Da
• NCBI Official Full Name: importin subunit alpha-1
• NCBI Official Synonym Full Names: karyopherin subunit alpha 2
• NCBI Official Symbol: KPNA2
• NCBI Official Synonym Symbols: QIP2; RCH1; IPOA1; SRP1alpha; SRP1-alpha
• NCBI Protein Information: importin subunit alpha-1
• UniProt Protein Name: Importin subunit alpha-1
• UniProt Gene Name: KPNA2
• UniProt Synonym Gene Names: RCH1; SRP1
• UniProt Entry Name: IMA1_HUMAN