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IHC (Immunohistchemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
Rabbit KRT5/CK5/Cytokeratin 5 Polyclonal Antibody | anti-KRT5 antibody
KRT5/CK5/Cytokeratin 5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Gene Names
KRT5; K5; CK5; DDD; EBS2; KRT5A
Reactivity
Mouse, Bovine, Rat, Pig, Human
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot
Purity
Immunoaffinity purified
Synonyms
KRT5/CK5/Cytokeratin 5; Polyclonal Antibody; KRT5/CK5/Cytokeratin 5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody; PathPlus KRT5/CK5/Cytokeratin 5 Antibody (C-Terminus); KRT5; 58 kd cytokeratin; 58 kDa cytokeratin; CK5; CK-5; Cytokeratin-5; DDD; EBS2; Keratin 5; KRT5A; Type-II keratin Kb5; Cytokeratin 5; K5; Keratin-5; anti-KRT5 antibody
Host
Rabbit
Reactivity
Mouse, Bovine, Rat, Pig, Human
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of Cytokeratin 5 protein.
Purity/Purification
Immunoaffinity purified
Form/Format
PBS, pH7.3, 0.01% Sodium Azide, 30% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-KRT5 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB)
Target
Human KRT5/CK5/Cytokeratin 5
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human Cytokeratin 5.
Conjugation
Unconjugated
Epitope
C-Terminus
Family
Keratin
Preparation and Storage
Store at -20 degree C. Aliquot to avoid freeze/thaw cycles.
IHC (Immunohistchemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistchemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
IHC (Immunohistochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))
WB (Western Blot)
(KRT5/CK5/Cytokeratin 5 Antibody-Western blot analysis of Cytokeratin 5 expression in DU145 (A); HeLa (B); MCF7 (C) whole cell lysates.)
WB (Western Blot)
(KRT5/CK5/Cytokeratin 5 Antibody-Western blot analysis of Cytokeratin 5 expression in DU145 (A); HeLa (B); MCF7 (C) whole cell lysates.)
ICC (Immunocytochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Immunofluorescent analysis of Cytokeratin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
ICC (Immunocytochemistry)
(KRT5/CK5/Cytokeratin 5 Antibody-Immunofluorescent analysis of Cytokeratin 5 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
Related Product Information for anti-KRT5 antibody
Cytokeratin 5 antibody is an unconjugated rabbit polyclonal antibody to Cytokeratin 5 (KRT5/CK5)(C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC and WB.
KRT5 (Cytokeratin 5, CK5) is a protein that dimerizes with keratin 14 to form the intermediate filament that form the cytoskeleton of basal epithelial cells. It is useful for identifying myoepithelial cells and basal cells in the breast, skin, salivary glands, and prostate. It is present in the skin, the mucosa of the oral cavity, oropharynx, hypopharynx, and larynx, and in mitotically active basal cells of the skin. It is commonly positive in squamous carcinomas of the head and neck, as well as in mesotheliomas. Mutations in KRT5 cause epidermolysis bullosa simplex. Commonly used with Cytokeratin 6, the CK5/6 marker is useful for the identification of mesotheliomas as well as identifying the presence of basal epithelium in benign glands versus malignant prostate and breast carcinomas, where the basal layer is lost.
KRT5 (Cytokeratin 5, CK5) is a protein that dimerizes with keratin 14 to form the intermediate filament that form the cytoskeleton of basal epithelial cells. It is useful for identifying myoepithelial cells and basal cells in the breast, skin, salivary glands, and prostate. It is present in the skin, the mucosa of the oral cavity, oropharynx, hypopharynx, and larynx, and in mitotically active basal cells of the skin. It is commonly positive in squamous carcinomas of the head and neck, as well as in mesotheliomas. Mutations in KRT5 cause epidermolysis bullosa simplex. Commonly used with Cytokeratin 6, the CK5/6 marker is useful for the identification of mesotheliomas as well as identifying the presence of basal epithelium in benign glands versus malignant prostate and breast carcinomas, where the basal layer is lost.
References
Int J Clin Exp pathol 2008 1(1):19; Histopathology 1997 31:140; J Cell Sci 1994 107(4):765
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
62,378 Da
NCBI Official Full Name
keratin, type II cytoskeletal 5
NCBI Official Synonym Full Names
keratin 5
NCBI Official Symbol
KRT5
NCBI Official Synonym Symbols
K5; CK5; DDD; EBS2; KRT5A
NCBI Protein Information
keratin, type II cytoskeletal 5; CK-5; cytokeratin-5; 58 kda cytokeratin; type-II keratin Kb5; epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types; keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockayne types)
UniProt Protein Name
Keratin, type II cytoskeletal 5
UniProt Gene Name
KRT5
UniProt Synonym Gene Names
CK-5; K5
UniProt Entry Name
K2C5_HUMAN
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Product Notes
The KRT5 krt5 (Catalog #AAA21347) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT5/CK5/Cytokeratin 5 Rabbit anti-Human Polyclonal (C-Terminus) Antibody reacts with Mouse, Bovine, Rat, Pig, Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT5/CK5/Cytokeratin 5 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Western Blot (WB). Researchers should empirically determine the suitability of the KRT5 krt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT5/CK5/Cytokeratin 5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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