Rabbit anti-Human, Mouse KRT8 Polyclonal Antibody | anti-KRT8 antibody

KRT8 Antibody

Cat. Num. AAA29673

• Gene Names: KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: KRT8, Antibody; KRT8 Antibody; KRT8; CARD2; CK8; CYK8; K2C8; anti-KRT8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total KRT8 protein.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 483

• Applicable Applications for anti-KRT8 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunohistochemistry: 1:50 - 1:200; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human KRT8.
• Target Name: KRT8
• Preparation and Storage: Store at -20 degree C

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cells using Cytokeratin-8 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of HeLa cell using KRT8 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Keratin 8 Antibody. The picture on the right is blocked with the synthesized peptide.)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.)

IHC (Immunohistchemistry)

(Immunohistochemistry of paraffin-embedded human esophagus using Cytokeratin-8 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human esophagus using Cytokeratin-8 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry)

(Immunohistochemical analysis of paraffin-embedded human lung cancer using KRT8 antibody at dilution of 1:200 (400x lens).)

IHC (Immunohistchemistry)

(Immunohistochemical analysis of paraffin-embedded human breast cancer using KRT8 antibody at dilution of 1:200 (400x lens).)

WB (Western Blot)

(Western blot analysis of lysates from HeLa cells, treated with Anisomycin 25ug/ml 30', using Keratin 8 Antibody. The lane on the right is blocked with the synthesized peptide.)

WB (Western Blot)

(Western Blot analysis of various cells using Cytokeratin 8 Polyclonal Antibody diluted at 1:500.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using Cytokeratin-8 at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts from normal (control) and Cytokeratin-8 knockout (KO) HeLa cells, using Cytokeratin-8 at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using KRT8 antibody.)

Related Product Information for anti-KRT8 antibody

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

Product Categories/Family for anti-KRT8 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 372466577
• NCBI GeneID: 3856
• NCBI Accession #: NP_001243222.1
• NCBI GenBank Nucleotide #: NM_001256293.1
• UniProt Accession #: P05787; Q14099; Q14716; Q14717; Q53GJ0; Q6DHW5; Q6GMY0; Q6P4C7; Q96J60; A8K4H3; B0AZN5; F8VXB4
• Molecular Weight: SDS-PAGE MW: 54KD
• NCBI Official Full Name: keratin, type II cytoskeletal 8 isoform 2
• NCBI Official Synonym Full Names: keratin 8, type II
• NCBI Official Symbol: KRT8
• NCBI Official Synonym Symbols: K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
• NCBI Protein Information: keratin, type II cytoskeletal 8
• UniProt Protein Name: Keratin, type II cytoskeletal 8
• UniProt Gene Name: KRT8
• UniProt Synonym Gene Names: CYK8; CK-8; K8
• UniProt Entry Name: K2C8_HUMAN