IHC (Immunohiostchemistry)
(LDB2 Antibody (C-term) (AAA286613)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LDB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit LDB2 Polyclonal Antibody | anti-LDB2 antibody
LDB2 Antibody (C-term)
Gene Names
LDB2; LDB1; CLIM1; LDB-2
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LDB2, Antibody; LDB2 Antibody (C-term); LIM domain-binding protein 2; LDB-2; Carboxyl-terminal LIM domain-binding protein 1; CLIM-1; LIM domain-binding factor CLIM1; LDB2; CLIM1; anti-LDB2 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Chicken, Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LDB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the C-terminal region of human LDB2.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.4 (varies by lot)
Sequence Positions
314-342
Sequence Length
347
Applicable Applications for anti-LDB2 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(LDB2 Antibody (C-term) (AAA286613)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LDB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(LDB2 Antibody (C-term) (AAA286613)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LDB2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(LDB2 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LDB2 antibody detected the LDB2 protein (arrow).)
WB (Western Blot)
(LDB2 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LDB2 antibody detected the LDB2 protein (arrow).)
Related Product Information for anti-LDB2 antibody
Genes encoding LIM domain-binding factors were initially
isolated in a screen for proteins that physically interact with the
LIM domains of nuclear proteins (summarized by Semina et al., 1998
[PubMed 9799849]). These proteins, such as the one encoded by the
LDB2 gene, are capable of binding to a variety of transcription
factors and are likely to function at enhancers to bring together
diverse transcription factors and form higher order activation
complexes or to block formation of such complexes (Jurata and Gill,
1997 [PubMed 9315627]). The family of genes encoding LIM
domain-binding factors includes 2 members isolated from the mouse,
Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli
(Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996
[PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their
homologs cloned from the frog, chicken, and fly. The fact that LIM
domain-binding factors are likely to be involved in the
coordination of the transcriptional activity of many diverse
factors might implicate them in human phenotypes characterized by
multiple affected sites.
isolated in a screen for proteins that physically interact with the
LIM domains of nuclear proteins (summarized by Semina et al., 1998
[PubMed 9799849]). These proteins, such as the one encoded by the
LDB2 gene, are capable of binding to a variety of transcription
factors and are likely to function at enhancers to bring together
diverse transcription factors and form higher order activation
complexes or to block formation of such complexes (Jurata and Gill,
1997 [PubMed 9315627]). The family of genes encoding LIM
domain-binding factors includes 2 members isolated from the mouse,
Clim1 (Bach et al., 1997 [PubMed 9192866]) and Clim2/Lbd1/Nli
(Agulnick et al., 1996 [PubMed 8918878]; Jurata et al., 1996
[PubMed 8876198]; Bach et al., 1997 [PubMed 9192866]) and their
homologs cloned from the frog, chicken, and fly. The fact that LIM
domain-binding factors are likely to be involved in the
coordination of the transcriptional activity of many diverse
factors might implicate them in human phenotypes characterized by
multiple affected sites.
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Hagg, S., et al. PLoS Genet. 5 (12), E1000754 (2009) :
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Kotaka, M., et al. J. Cell. Biochem. 78(4):558-565(2000)
Bach, I., et al. Nat. Genet. 22(4):394-399(1999)
Hagg, S., et al. PLoS Genet. 5 (12), E1000754 (2009) :
Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
Kotaka, M., et al. J. Cell. Biochem. 78(4):558-565(2000)
Bach, I., et al. Nat. Genet. 22(4):394-399(1999)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42793
NCBI Official Full Name
LIM domain-binding protein 2 isoform b
NCBI Official Synonym Full Names
LIM domain binding 2
NCBI Official Symbol
LDB2
NCBI Official Synonym Symbols
LDB1; CLIM1; LDB-2
NCBI Protein Information
LIM domain-binding protein 2
UniProt Protein Name
LIM domain-binding protein 2
UniProt Gene Name
LDB2
UniProt Synonym Gene Names
CLIM1; LDB-2; CLIM-1
UniProt Entry Name
LDB2_HUMAN
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Product Notes
The LDB2 ldb2 (Catalog #AAA286613) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 314-342. The LDB2 Antibody (C-term) reacts with Human (Predicted Reactivity: Chicken, Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's LDB2 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LDB2 ldb2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LDB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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