IF (Immunofluorescence)
(Fluorescent confocal image of A431 cell stained with LEF1 Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LEF1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). LEF1 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)
Rabbit LEF1 Polyclonal Antibody | anti-LEF1 antibody
LEF1 Antibody (N-term)
Gene Names
LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Applications
Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LEF1, Antibody; LEF1 Antibody (N-term); Lymphoid enhancer-binding factor 1; LEF-1; T cell-specific transcription factor 1-alpha; TCF1-alpha; LEF1; anti-LEF1 antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LEF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-37 amino acids from the N-terminal region of human LEF1.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
10-37
Sequence Length
303
Applicable Applications for anti-LEF1 antibody
IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IF (Immunofluorescence)
(Fluorescent confocal image of A431 cell stained with LEF1 Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LEF1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). LEF1 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)
IF (Immunofluorescence)
(Fluorescent confocal image of A431 cell stained with LEF1 Antibody (N-term). A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with LEF1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). LEF1 immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)
WB (Western Blot)
(LEF1 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the LEF1 antibody detected the LEF1 protein (arrow).)
WB (Western Blot)
(LEF1 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the LEF1 antibody detected the LEF1 protein (arrow).)
Related Product Information for anti-LEF1 antibody
This gene encodes a transcription factor belonging to a
family of proteins that share homology with the high mobility group
protein-1. The protein encoded by this gene can bind to a
functionally important site in the T-cell receptor-alpha enhancer,
thereby conferring maximal enhancer activity. This transcription
factor is involved in the Wnt signaling pathway, and it may
function in hair cell differentiation and follicle morphogenesis.
Mutations in this gene have been found in somatic sebaceous tumors.
This gene has also been linked to other cancers, including
androgen-independent prostate cancer. Alternative splicing results
in multiple transcript variants.
family of proteins that share homology with the high mobility group
protein-1. The protein encoded by this gene can bind to a
functionally important site in the T-cell receptor-alpha enhancer,
thereby conferring maximal enhancer activity. This transcription
factor is involved in the Wnt signaling pathway, and it may
function in hair cell differentiation and follicle morphogenesis.
Mutations in this gene have been found in somatic sebaceous tumors.
This gene has also been linked to other cancers, including
androgen-independent prostate cancer. Alternative splicing results
in multiple transcript variants.
Product Categories/Family for anti-LEF1 antibody
References
Gutierrez, A. Jr., et al. Blood 116(16):2975-2983(2010)
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Chen, Q.Y., et al. J. Immunol. 184(9):5047-5054(2010)
Beagle, B., et al. PLoS ONE 5 (7), E11821 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Chen, Q.Y., et al. J. Immunol. 184(9):5047-5054(2010)
Beagle, B., et al. PLoS ONE 5 (7), E11821 (2010) :
Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
44201
NCBI Official Full Name
lymphoid enhancer-binding factor 1 isoform 2
NCBI Official Synonym Full Names
lymphoid enhancer-binding factor 1
NCBI Official Symbol
LEF1
NCBI Official Synonym Symbols
LEF-1; TCF10; TCF7L3; TCF1ALPHA
NCBI Protein Information
lymphoid enhancer-binding factor 1
UniProt Protein Name
Lymphoid enhancer-binding factor 1
UniProt Gene Name
LEF1
UniProt Synonym Gene Names
LEF-1; TCF1-alpha
UniProt Entry Name
LEF1_HUMAN
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Product Notes
The LEF1 lef1 (Catalog #AAA290121) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 10-37. The LEF1 Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's LEF1 can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LEF1 lef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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