Rabbit LEO1 Polyclonal Antibody | anti-LEO1 antibody

LEO1 Polyclonal Antibody

Cat. Num. AAA22291

• Gene Names: LEO1; RDL
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity purification
• Synonyms: LEO1, Antibody; LEO1 Polyclonal Antibody; LEO1; RDL; anti-LEO1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.01% thiomersal,50% glycerol,pH7.3.
• Concentration: 1mg/mL (varies by lot)

• Applicable Applications for anti-LEO1 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Recombinant fusion protein of human LEO1
• Research Area: Cancer, Epigenetics and Nuclear Signaling
• Preparation and Storage: Storage: -20 degree C/One year. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of U2OS cells using LEO1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using LEO1 Polyclonal Antibody at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded mouse liver using LEO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human esophageal cancer using LEO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry)

(Immunohistochemistry of paraffin-embedded human colon carcinoma using LEO1 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using LEO1 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines using LEO1 Polyclonal Antibody at 1:1000 dilution.)

Related Product Information for anti-LEO1 antibody

LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]

Product Categories/Family for anti-LEO1 antibody

Antibodies; Primary Antibodies; Polyclonal Antibody

NCBI and Uniprot Product Information

• NCBI GI #: 20270337
• NCBI GeneID: 123169
• NCBI Accession #: NP_620147.1
• NCBI GenBank Nucleotide #: NM_138792.3
• UniProt Accession #: Q8WVC0; Q96N99
• Molecular Weight: 68,004 Da
• NCBI Official Full Name: RNA polymerase-associated protein LEO1 isoform 1
• NCBI Official Synonym Full Names: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• NCBI Official Symbol: LEO1
• NCBI Official Synonym Symbols: RDL
• NCBI Protein Information: RNA polymerase-associated protein LEO1; replicative senescence down-regulated leo1-like protein
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• UniProt Gene Name: LEO1
• UniProt Synonym Gene Names: RDL
• UniProt Entry Name: LEO1_HUMAN