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FCM/FACS (Flow Cytometry)
(LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit LEP Polyclonal Antibody | anti-LEP antibody
LEP Antibody (N-term)
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunofluorescence, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LEP, Antibody; LEP Antibody (N-term); Leptin; Obese protein; Obesity factor; LEP; OB; OBS; anti-LEP antibody
Host
Rabbit
Reactivity
Human (Predicted Reactivity: Mouse)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-37 amino acids from the N-terminal region of human LEP.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
8-37
Sequence Length
167
Applicable Applications for anti-LEP antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(LEP Antibody (N-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohistochemistry)
(LEP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(LEP Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of LEP Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of LEP Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
IF (Immunofluorescence)
(Confocal immunofluorescent analysis of LEP Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
WB (Western Blot)
(Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.)
WB (Western Blot)
(Western blot analysis of LEP (arrow) using rabbit polyclonal LEP Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the LEP gene.)
WB (Western Blot)
(LEP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).)
WB (Western Blot)
(LEP Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the LEP antibody detected the LEP protein (arrow).)
Related Product Information for anti-LEP antibody
This gene encodes a protein that is secreted by white
adipocytes, and which plays a major role in the regulation of body
weight. This protein, which acts through the leptin receptor,
functions as part of a signaling pathway that can inhibit food
intake and/or regulate energy expenditure to maintain constancy of
the adipose mass. This protein also has several endocrine
functions, and is involved in the regulation of immune and
inflammatory responses, hematopoiesis, angiogenesis and wound
healing. Mutations in this gene and/or its regulatory regions cause
severe obesity, and morbid obesity with hypogonadism. This gene has
also been linked to type 2 diabetes mellitus development. [provided
by RefSeq].
adipocytes, and which plays a major role in the regulation of body
weight. This protein, which acts through the leptin receptor,
functions as part of a signaling pathway that can inhibit food
intake and/or regulate energy expenditure to maintain constancy of
the adipose mass. This protein also has several endocrine
functions, and is involved in the regulation of immune and
inflammatory responses, hematopoiesis, angiogenesis and wound
healing. Mutations in this gene and/or its regulatory regions cause
severe obesity, and morbid obesity with hypogonadism. This gene has
also been linked to type 2 diabetes mellitus development. [provided
by RefSeq].
Product Categories/Family for anti-LEP antibody
References
Romero, R., et al. Am. J. Obstet. Gynecol. 203 (4), 361 (2010) :
Qin, J., et al. J. Hum. Genet. 55(10):704-706(2010)
Friedlander, Y., et al. Ann. Hum. Genet. 74(5):387-398(2010)
Sarzynski, M.A., et al. Int J Obes (Lond) (2010) In press :
Iciek, R., et al. Ginekol. Pol. 81(8):571-577(2010)
Qin, J., et al. J. Hum. Genet. 55(10):704-706(2010)
Friedlander, Y., et al. Ann. Hum. Genet. 74(5):387-398(2010)
Sarzynski, M.A., et al. Int J Obes (Lond) (2010) In press :
Iciek, R., et al. Ginekol. Pol. 81(8):571-577(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
18641
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
LEP
NCBI Official Synonym Symbols
OB; OBS; LEPD
NCBI Protein Information
leptin
UniProt Protein Name
Leptin
UniProt Gene Name
LEP
UniProt Synonym Gene Names
OB; OBS
UniProt Entry Name
LEP_HUMAN
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Product Notes
The LEP lep (Catalog #AAA284107) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 8-37. The LEP Antibody (N-term) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's LEP can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), IF (Immunofluorescence), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LEP lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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