Rabbit anti-Mouse, Rat Leukocyte Elastase Inhibitor Polyclonal Antibody | anti-LEI antibody

Polyclonal Antibody to Leukocyte Elastase Inhibitor (LEI)

Cat. Num. AAA20836

• Gene Names: Serpinb1a; Serpinb1
• Reactivity: Mouse, Rat
• Applications: Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Leukocyte Elastase Inhibitor, Antibody; Polyclonal Antibody to Leukocyte Elastase Inhibitor (LEI); anti-LEI antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Liquid; Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 0.5mg/mL (varies by lot)

• Applicable Applications for anti-LEI antibody: Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC) / Immunofluorescence (IF)
• Application Notes: Western blotting: 0.01-3ug/mL;; Immunohistochemistry: 5-20ug/mL;; Immunocytochemistry: 5-20ug/mL;; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Immunogen: Recombinant LEI (His17~Glu332 (Accession # Q4G075)) expressed in E.coli
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4ºC for frequent use.; Aliquot and store at -20ºC for 24 months.; ; Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

IF (Immunofluorescence)

(FITC staining on IF; Sample: Hela cellPrimary Ab: 10ug/ml Rabbit Anti-Rat LEI AntibodySecond Ab: 2ug/ml FITC-LinkedCaprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: Please Inquire))

IF (Immunofluorescence)

(FITC staining on IF;Samples: Human Hela Cells;Primary Ab: 10ug/ml Rabbit Anti-Rat LEIAntibody Second Ab: 1ug/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: Rat Spleen TissuePrimary Ab: 10ug/ml Rabbit Anti-Rat LEIAntibody Control:Used PBS instead of primary antibodyAntibody Second Ab:2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Sample: Rat Lung TissuePrimary Ab: 10ug/ml Rabbit Anti-Rat LEIAntibody Control: Used PBS instead of primary antibodyAntibody Second Ab:2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistchemistry)

(DAB staining on IHC-P;Samples: Rat Kidney Tissue;Primary Ab: 10ug/ml Rabbit Anti-Rat LEIAntibody Second Ab:2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry)

(DAB staining on IHC-P;Samples: Rat Spleen TissuePrimary Ab: 10ug/ml Rabbit Anti-Rat LEIAntibody Second Ab:2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry)

(Dab staining on IHC-P:Samples: Rat Pancreas Tissue; Primary Ab: 10ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistchemistry)

(Dab staining on IHC-P;Samples:Rat Lung tissuePrimary Ab:10ug/ml Rabbit Anti-Rat LEI AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Rat Pancreas Tissue)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Rat Liver Tissue)

IHC (Immunohistochemistry)

(DAB staining on IHC-P; Samples: Rat Spleen Tissue.)

WB (Western Blot)

(Western Blot: Sample: Recombinant LEI, Rat.)

WB (Western Blot)

(Western Blot; Sample: Rat Heart lysatePrimary Ab: 3ug/ml Rabbit Anti-Rat LEIAntibody Second Ab: 0.2ug/ml HRP-LinkedCaprine Anti-Rabbit IgG Polyclonal Anitbody)

NCBI and Uniprot Product Information

• NCBI GI #: 72255515
• NCBI GeneID: 291091
• NCBI Accession #: NP_001026812.1
• NCBI GenBank Nucleotide #: NM_001031642.1
• UniProt Accession #: Q4G075
• NCBI Official Full Name: leukocyte elastase inhibitor A
• NCBI Official Synonym Full Names: serpin family B member 1A
• NCBI Official Symbol: Serpinb1a
• NCBI Official Synonym Symbols: Serpinb1
• NCBI Protein Information: leukocyte elastase inhibitor A
• UniProt Protein Name: Leukocyte elastase inhibitor A
• UniProt Gene Name: Serpinb1a