Application Data
(ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.)
Rabbit anti-Human, Mouse LGR5 Polyclonal Antibody | anti-LGR5 antibody
LGR5 Antibody
Gene Names
LGR5; FEX; HG38; GPR49; GPR67; GRP49
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry
Purity
Immunogen affinity purified
Synonyms
LGR5, Antibody; LGR5 Antibody; anti-LGR5 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of LGR5.
Purity/Purification
Immunogen affinity purified
Form/Format
Liquid; 1*TBS (pH7.4), 0.5% BSA, 25% Glycerol, Preservative: 0.05% Sodium Azide.
Sequence Length
883
Applicable Applications for anti-LGR5 antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
WB: 1:1,000-1:2,000
ICC: 1:50
ICC: 1:50
Cellular Localization
Cell Membrane Cell nucleus
Positive Control
F9, NCCIT
Preparation and Storage
Store at +4 degree C after thawing. Aliquot store at-20 degree C or-80 degree C. Avoid repeated freeze/thaw cycles.
Application Data
(ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.)
Application Data
(ICC image of LGR5 antibody stained NCCIT cells. The secondary antibody (green) was goat anti-rabbit IgG (H+L) FITC conjugated.)
WB (Western Blot)
(Western blot analysis on F9 (A)and NCCIT (B) cell lysates using anti-LGR5 rabbit polyclonal antibody.)
WB (Western Blot)
(Western blot analysis on F9 (A)and NCCIT (B) cell lysates using anti-LGR5 rabbit polyclonal antibody.)
Related Product Information for anti-LGR5 antibody
G-protein coupled receptor GPR49 (also known as Leucine-rich repeat-containing G-protein coupled receptor 5, Lgr5) is a stem cell marker of the intestinal epithelium and the hair follicule. It is expressed in the gonads, the adrenal gland, and in the brain, overexpressed in cancers of the ovary, colon and liver.
Product Categories/Family for anti-LGR5 antibody
References
1. Identification of overexpression of orphan G protein-coupled receptor GPR49 in human colon and ovarian primary tumors."McClanahan T., Koseoglu S., Smith K., Grein J., Gustafson E., Black S., Kirschmeier P., Samatar A.A.Cancer Biol. Ther. 5:419-426(2006). 2. "Immunostaining of Lgr5, an intestinal stem cell marker, in normal and premalignant human gastrointestinal tissue."Becker L., Huang Q., Mashimo H.ScientificWorldJournal 8:1168-1176(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
95kDa
NCBI Official Full Name
leucine-rich repeat-containing G-protein coupled receptor 5 isoform 2
NCBI Official Synonym Full Names
leucine rich repeat containing G protein-coupled receptor 5
NCBI Official Symbol
LGR5
NCBI Official Synonym Symbols
FEX; HG38; GPR49; GPR67; GRP49
NCBI Protein Information
leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Protein Name
Leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Gene Name
LGR5
UniProt Synonym Gene Names
GPR49; GPR67
Similar Products
Product Notes
The LGR5 lgr5 (Catalog #AAA17985) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LGR5 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LGR5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). WB: 1:1,000-1:2,000 ICC: 1:50. Researchers should empirically determine the suitability of the LGR5 lgr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LGR5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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