Rabbit LIN7A Polyclonal Antibody | anti-LIN7A antibody

Anti-LIN7A Antibody, Rabbit Polyclonal

Cat. Num. AAA255101

• Gene Names: LIN7A; LIN7; VELI1; LIN-7A; MALS-1; TIP-33
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovin (Species predicted to react based on 100% sequence homology)
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: LIN7A, Antibody; Anti-LIN7A Antibody, Rabbit Polyclonal; LIN7A Antibody, Rabbit PAb, Antigen Affinity Purified; lin-7 homolog A (C. elegans); Anti-LIN-7A Antibody; Anti-LIN7 Antibody; Anti-MALS-1 Antibody; Anti-TIP-33 Antibody; Anti-VELI1 Antibody; anti-LIN7A antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovin (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LIN7A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LIN7A antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), WB (Western Blot)
• Immunogen: A synthetic peptide corresponding to the center region of the Human LIN7A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human LIN7A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(ATP6V1C1 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell Lysate4 uL anti-ATP6V1C1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ATP6V1C1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 44 kDaObserved band size :45 kDa)

IF (Immunofluorescence)

(Immunofluorescence staining of LIN7A in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human LIN7A polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.)

WB (Western Blot)

(Anti-LIN7A rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse brain tissue lysateLane B: Rat brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:26 kDa)

Related Product Information for anti-LIN7A antibody

LIN7A (Lin-7 Homolog A, Crumbs Cell Polarity Complex Component) is a Protein Coding gene. LIN7A encodes a scaffold protein that is important for synaptic function, as a possible responsible gene for ID. The encoded protein also is required for the localization of some specific channels and can be part of a protein complex that couples synaptic vesicle exocytosis to cell adhesion in the brain. LIN7A is a candidate gene responsible for 12q21-deletion syndrome, and abnormal neuronal migration and interhemispheric axon development may contribute to ID and corpus callosum hypoplasia, respectively. LIN7A has a crucial role in the polarity abnormalities associated with breast carcinogenesis. Diseases associated with LIN7A include Median Arcuate Ligament Syndrome and Pulmonary Aspergilloma.

NCBI and Uniprot Product Information

• NCBI GI #: 4759306
• NCBI GeneID: 8825
• NCBI Accession #: NP_004655.1
• NCBI GenBank Nucleotide #: NM_004664.2
• UniProt Accession #: O14910; Q147W1; Q6LES3; Q7LDS4; A4FTY3
• Molecular Weight: 25,997 Da
• NCBI Official Full Name: protein lin-7 homolog A
• NCBI Official Synonym Full Names: lin-7 homolog A (C. elegans)
• NCBI Official Symbol: LIN7A
• NCBI Official Synonym Symbols: LIN7; VELI1; LIN-7A; MALS-1; TIP-33
• NCBI Protein Information: protein lin-7 homolog A; hLin-7; mammalian LIN-7 1; mammalian lin-seven protein 1; tax interaction protein 33; veli-1; vertebrate LIN7 homolog 1; vertebrate lin-7 homolog 1
• UniProt Protein Name: Protein lin-7 homolog A
• UniProt Gene Name: LIN7A
• UniProt Synonym Gene Names: MALS1; VELI1; Lin-7A; hLin-7; MALS-1; TIP-33; Veli-1
• UniProt Entry Name: LIN7A_HUMAN