Standard Curve (Sample)
(Fig.1. Human Retinoic acid receptor alpha (RARA) Standard Curve.)
Human Retinoic Acid Receptor alpha (RARA) ELISA Kit | RARA elisa kit
Human Retinoic Acid Receptor alpha (RARA) ELISA Kit
Gene Names
RARA; RAR; NR1B1
Reactivity
Human
Synonyms
Retinoic Acid Receptor alpha (RARA); N/A; Human Retinoic Acid Receptor alpha (RARA) ELISA Kit; RARA; NR1B1; RAR; Retinoic acid receptor; alpha polypeptide; nucleophosmin-retinoic acid receptor alpha fusion protein NPM-RAR long form; RARA elisa kit
Reactivity
Human
Specificity
Human Retinoic acid receptor alpha (RARA) ELISA Kit has high sensitivity and excellent specificity for detection of Human RARA. No significant cross-reactivity or interference between Human RARA and analogues was observed.
Sequence Length
462
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Standard Curve (Sample)
(Fig.1. Human Retinoic acid receptor alpha (RARA) Standard Curve.)
Standard Curve (Sample)
(Fig.1. Human Retinoic acid receptor alpha (RARA) Standard Curve.)
Related Product Information for RARA elisa kit
Description: This Human Retinoic acid receptor alpha (RARA) ELISA Kit employs a two-site sandwich ELISA to quantitate RARA in samples. An antibody specific for RARA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any RARA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RARA is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RARA bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The retinoic acid receptor (RAR) is a type of nuclear receptor which is activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-alpha, RAR-beta, and RAR-gamma encoded by the RARA, RARB, RARG genes respectively. Each receptor isoform has several splice variants: two- for alpha, four- for beta and two- for gamma.As with other type II nuclear receptors, RAR heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements complexed with corepressor protein. Binding of agonist ligands to RAR results in dissociation of corepressor and recruitment of coactivator protein which in turn promotes transcription of the downstream target gene into mRNA and eventually protein.
Background: The retinoic acid receptor (RAR) is a type of nuclear receptor which is activated by both all-trans retinoic acid and 9-cis retinoic acid. There are three retinoic acid receptors (RAR), RAR-alpha, RAR-beta, and RAR-gamma encoded by the RARA, RARB, RARG genes respectively. Each receptor isoform has several splice variants: two- for alpha, four- for beta and two- for gamma.As with other type II nuclear receptors, RAR heterodimerizes with RXR and in the absence of ligand, the RAR/RXR dimer binds to hormone response elements complexed with corepressor protein. Binding of agonist ligands to RAR results in dissociation of corepressor and recruitment of coactivator protein which in turn promotes transcription of the downstream target gene into mRNA and eventually protein.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
retinoic acid receptor alpha isoform 1
NCBI Official Synonym Full Names
retinoic acid receptor alpha
NCBI Official Symbol
RARA
NCBI Official Synonym Symbols
RAR; NR1B1
NCBI Protein Information
retinoic acid receptor alpha
UniProt Protein Name
Retinoic acid receptor alpha
UniProt Gene Name
RARA
UniProt Synonym Gene Names
NR1B1; RAR-alpha
UniProt Entry Name
RARA_HUMAN
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Product Notes
The Human RARA rara (Catalog #AAA331161) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA331161 ELISA Kit recognizes Human RARA. It is sometimes possible for the material contained within the vial of "Retinoic Acid Receptor alpha (RARA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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