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IF (Immunofluorescence) (Immunofluorescence analysis of U-2OS cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

Rabbit Lipoprotein lipase (LPL) Polyclonal Antibody | anti-LPL antibody

Lipoprotein lipase (LPL) Rabbit pAb

Gene Names
LPL; LIPD; HDLCQ11
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Synonyms
Lipoprotein lipase (LPL); Polyclonal Antibody; Lipoprotein lipase (LPL) Rabbit pAb; LPL; HDLCQ11; LIPD; anti-LPL antibody
Ordering
For Research Use Only!
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Applicable Applications for anti-LPL antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IF: 1:50-1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 124-475 of human Lipoprotein lipase (Lipoprotein lipase (LPL))(NP_000228.1).
Species Reactivity/Cross-Reactivity
Human, Mouse, Rat
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.

IF (Immunofluorescence)

(Immunofluorescence analysis of U-2OS cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2OS cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of NIH/3T3 cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence)

(Immunofluorescence analysis of C6 cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot)

(Western blot analysis of extracts of various cell lines, using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Lipoprotein lipase (Lipoprotein lipase (LPL)) antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
Related Product Information for anti-LPL antibody
Background: LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed MW: 53kDa
Calculated MW: 53kDa
NCBI Official Full Name
lipoprotein lipase
NCBI Official Synonym Full Names
lipoprotein lipase
NCBI Official Symbol
LPL
NCBI Official Synonym Symbols
LIPD; HDLCQ11
NCBI Protein Information
lipoprotein lipase
UniProt Protein Name
Lipoprotein lipase
UniProt Gene Name
LPL
UniProt Synonym Gene Names
LIPD; LPL
UniProt Entry Name
LIPL_HUMAN

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Product Notes

The LPL lpl (Catalog #AAA28325) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lipoprotein lipase (LPL) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:2000 IF: 1:50-1:200. Researchers should empirically determine the suitability of the LPL lpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipoprotein lipase (LPL), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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