WB (Western Blot)
(Detection of human LOK by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-LOK antibody AAA210928 (lot AAA210928-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human LOK Polyclonal Antibody | anti-LOK antibody
Rabbit anti-LOK Antibody, Affinity Purified
Gene Names
STK10; LOK; PRO2729
Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
LOK, Antibody; Rabbit anti-LOK Antibody, Affinity Purified; serine/threonine-protein kinase 10; LOK; lymphocyte-oriented kinase; PRO2729; Serine/threonine-protein kinase 10; serine/threonine kinase 10; anti-LOK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-LOK antibody
IP (Immunoprecipitation), WB (Western Blot)
Immunogen
Between 325 and 375
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human LOK by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-LOK antibody AAA210928 (lot AAA210928-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human LOK by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-LOK antibody AAA210928 (lot AAA210928-2) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human LOK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-LOK antibody AAA210928 (lot AAA210928-2) used for IP at 6 ug/mg lysate. LOK was also immunoprecipitated by rabbit anti-LOK antibody For blotting immunoprecipitated LOK, AAA210928 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation)
(Detection of human LOK by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-LOK antibody AAA210928 (lot AAA210928-2) used for IP at 6 ug/mg lysate. LOK was also immunoprecipitated by rabbit anti-LOK antibody For blotting immunoprecipitated LOK, AAA210928 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)
Related Product Information for anti-LOK antibody
LOK is a member of the Ste20 family of serine-threonine protein kinases. LOK associates with polo-like 1 kinase (PLK1) and may function to regulate its kinase activity. LOK has also been demonstrated to be involved in the regulation of the CD28 response-element in T-cells via the mitogen activated protein kinase kinase 1 pathway.
Product Categories/Family for anti-LOK antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,135 Da
NCBI Official Full Name
serine/threonine-protein kinase 10
NCBI Official Synonym Full Names
serine/threonine kinase 10
NCBI Official Symbol
STK10
NCBI Official Synonym Symbols
LOK; PRO2729
NCBI Protein Information
serine/threonine-protein kinase 10; lymphocyte-oriented kinase
UniProt Protein Name
Serine/threonine-protein kinase 10
UniProt Gene Name
STK10
UniProt Synonym Gene Names
LOK
UniProt Entry Name
STK10_HUMAN
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Product Notes
The LOK stk10 (Catalog #AAA210928) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LOK Antibody, Affinity Purified reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LOK can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LOK stk10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LOK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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