IP (Immunoprecipitation)
(STK10 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Raji Whole Cell Lysate4 uL anti-STK10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-STK10 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 130 kDaObserved band size :130 kDa)
Rabbit anti-Human LOK Polyclonal Antibody | anti-LOK antibody
Anti-LOK Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunoprecipitation, ELISA, Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
LOK, Antibody; Anti-LOK Antibody, Rabbit Polyclonal; serine/threonine kinase 10; Anti-LOK Antibody; Anti-PRO2729 Antibody; anti-LOK antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human LOK
Purity/Purification
Protein A & Antigen Affinity
Form/Format
0.2um filtered solution in PBS
Applicable Applications for anti-LOK antibody
IP (Immunoprecipitation), ELISA, WB (Western Blot)
Immunogen
Recombinant Human LOK Protein
Preparation
Produced in rabbits immunized with purified, recombinant Human LOK (rh LOK). LOK specific IgG was purified by Human LOK affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
IP (Immunoprecipitation)
(STK10 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Raji Whole Cell Lysate4 uL anti-STK10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-STK10 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 130 kDaObserved band size :130 kDa)
IP (Immunoprecipitation)
(STK10 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell LysateLane B:0.5 mg Hela Whole Cell LysateLane C:0.5 mg Raji Whole Cell Lysate4 uL anti-STK10 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-STK10 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 130 kDaObserved band size :130 kDa)
WB (Western Blot)
(Anti-STK10 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: A431 Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:112 kDaObserved band size:140 kDa)
WB (Western Blot)
(Anti-STK10 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HeLa Whole Cell LysateLane C: A431 Whole Cell LysateLane D: Raji Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H&L) /Dylight 800 at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:112 kDaObserved band size:140 kDa)
Related Product Information for anti-LOK antibody
Serine/threonine-protein kinase 1, also known as Lymphocyte-oriented kinase, STK1 and LOK, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE2 subfamily. Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells. The Ste2 family of serine/threonine kinases plays an important role in numerous cellular functions such as growth, apoptosis, and morphogenesis. STK1 is similar to several known polo-like kinase kinases. It can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. STK1 can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. Stk1 can associate with Plk1 in cells and furthermore can phosphorylate Plk1. It can also act on substrates such as myelin basic protein and histone 2A on serine and threonine residues.
References
Kuramochi S, et al., 1997, J. Biol. Chem. 272 (36): 22679-84.
Kuramochi S, et al., 1999, Immunogenetics. 49 (5): 369-75.
Walter, SA. et al., J Biol Chem 2003, 278 (20):18221-8.
Kuramochi S, et al., 1999, Immunogenetics. 49 (5): 369-75.
Walter, SA. et al., J Biol Chem 2003, 278 (20):18221-8.
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Product Notes
The LOK (Catalog #AAA258898) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LOK Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LOK can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LOK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LOK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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