IHC (Immunohiostchemistry)
(Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.)
Rabbit LRRC8A Polyclonal Antibody | anti-LRRC8A antibody
LRRC8A Antibody
Gene Names
LRRC8A; AGM5; LRRC8; SWELL1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot, ELISA
Purity
Affinity chromatography purified via peptide column.
Synonyms
LRRC8A, Antibody; LRRC8A Antibody; Leucine rich repeat containing 8 family member A, leucine-rich repeat-containing protein 8A, LRRC8, AGM5; anti-LRRC8A antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
LRRC8A antibody is human, mouse and rat reactive.
Purity/Purification
Affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
810
Applicable Applications for anti-LRRC8A antibody
IHC (Immunohistochemistry), WB (Western Blot), ELISA
Immunogen
LRRC8A antibody was raised against a 15 amino acid peptide near the amino terminus of human LRRC8A.
Buffer
Supplied in PBS containing 0.02% sodium azide.
Positive Control 1
EL4 Cell Lysate
Conjugate
Unconjugated
MW Explanation
Multiple other LRRC8A antibodies also show a ~95kD band
Preparation and Storage
Store at 4 degree C for three months and -20 degree C, stable for up to one year.
IHC (Immunohiostchemistry)
(Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.)
IHC (Immunohiostchemistry)
(Immunohistochemistry of LRRC8A in rat spleen tissue with LRRC8A antibody at 2.5 μg/mL.)
WB (Western Blot)
(Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.)
WB (Western Blot)
(Western blot analysis of LRRC8A in EL4 cell lysate with LRRC8A antibody at (A) 1 and (B) 2 μg/ml.)
Related Product Information for anti-LRRC8A antibody
The leucine rich repeat containing 8 family member A protein (LRRC8A) belongs the LRRC8 family of proteins within the LRR superfamily of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions (1,2). LRRC8A is a four-pass transmembrane protein that plays a role in B cell development. Defects in this gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation (1). LRRC8A has also been shown to be an essential component of the volume-regulated anion channel VRAC (3).
Product Categories/Family for anti-LRRC8A antibody
References
Sawada A, Takihara Y, Kim JY, et al. A congenital mutation of the novel gene LRRC8 causes agammaglobulinemia in humans. J. Clin. Invest. 2003; 112:1707-13. Kubota K, Kim JY, Sawada A, et al. LRRC8 involved in B cell development belongs to a novel family of leucine-rich repeat proteins. FEBS Lett. 2004; 564:147-52. Voss FK, Ullrich F, Munch J, et al. Identification of LRRC8 heteromers as an essential component of the volume-regulated anion channel VRAC. Science 2014; 344:634-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted: 89 kDa
Observed: 95 kDa
Observed: 95 kDa
NCBI Official Full Name
volume-regulated anion channel subunit LRRC8A
NCBI Official Synonym Full Names
leucine-rich repeat containing 8 family member A
NCBI Official Symbol
LRRC8A
NCBI Official Synonym Symbols
AGM5; LRRC8; SWELL1
NCBI Protein Information
volume-regulated anion channel subunit LRRC8A
UniProt Protein Name
Volume-regulated anion channel subunit LRRC8A
UniProt Gene Name
LRRC8A
UniProt Synonym Gene Names
KIAA1437; LRRC8; SWELL1
UniProt Entry Name
LRC8A_HUMAN
Similar Products
Product Notes
The LRRC8A lrrc8a (Catalog #AAA40828) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LRRC8A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LRRC8A can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), ELISA. Researchers should empirically determine the suitability of the LRRC8A lrrc8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRC8A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.