WB (Western Blot)
(Detection of human and mouse LRRC8A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody AAA212530 (lot AAA212530-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
Rabbit anti-Human, Mouse LRRC8A Polyclonal Antibody | anti-LRRC8A antibody
Rabbit anti-LRRC8A Antibody, Affinity Purified
Gene Names
LRRC8A; AGM5; LRRC8
Reactivity
Human, Mouse
Applications
Immunoprecipitation, Western Blot
Purity
Antigen Affinity Purified
Synonyms
LRRC8A, Antibody; Rabbit anti-LRRC8A Antibody, Affinity Purified; SWELL1; AGM5; volume-regulated anion channel subunit LRRC8A; swelling protein 1; LRRC8; leucine rich repeat containing 8 family member A; leucine-rich repeat-containing protein 8A; Volume-regulated anion channel subunit LRRC8A; leucine rich repeat containing 8 VRAC subunit A; anti-LRRC8A antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid. Whole IgG. Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Concentration
1000ug/ml (varies by lot)
Applicable Applications for anti-LRRC8A antibody
IP (Immunoprecipitation), WB (Western Blot)
Predicted Reactivity
Rat
Immunogen
Between 760 and C-terminus
Conjugation
Unconjugated
Preparation and Storage
Store at 2-8 degree C
Shelf Life: 1 year from date of receipt
Shelf Life: 1 year from date of receipt
WB (Western Blot)
(Detection of human and mouse LRRC8A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody AAA212530 (lot AAA212530-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
WB (Western Blot)
(Detection of human and mouse LRRC8A by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody AAA212530 (lot AAA212530-1) used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)
IP (Immunoprecipitation)
(Detection of human LRRC8A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody AAA212530 (lot AAA212530-1) used for IP at 6 ug/mg lysate. LRRC8A was also immunoprecipitated by rabbit anti-LRRC8A antibodies BL15169 and For blotting immunoprecipitated LRRC8A, AAA212530 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IP (Immunoprecipitation)
(Detection of human LRRC8A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-LRRC8A antibody AAA212530 (lot AAA212530-1) used for IP at 6 ug/mg lysate. LRRC8A was also immunoprecipitated by rabbit anti-LRRC8A antibodies BL15169 and For blotting immunoprecipitated LRRC8A, AAA212530 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)
Related Product Information for anti-LRRC8A antibody
LRRC8A belongs to the leucine-rich repeat family of proteins, which are involved in diverse biological processes, including cell adhesion, cellular trafficking, and hormone-receptor interactions. LRRC8A is a putative four-pass transmembrane protein that plays a role in B cell development. Defects in the LRRC8A gene cause autosomal dominant non-Bruton type agammaglobulinemia, an immunodeficiency disease resulting from defects in B cell maturation [taken from NCBI Entrez Gene (Gene ID: 56262)].
Product Categories/Family for anti-LRRC8A antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94,199 Da
NCBI Official Full Name
volume-regulated anion channel subunit LRRC8A
NCBI Official Synonym Full Names
leucine rich repeat containing 8 family, member A
NCBI Official Symbol
LRRC8A
NCBI Official Synonym Symbols
AGM5; LRRC8
NCBI Protein Information
volume-regulated anion channel subunit LRRC8A; swelling protein 1; leucine-rich repeat-containing protein 8A
UniProt Protein Name
Volume-regulated anion channel subunit LRRC8A
UniProt Gene Name
LRRC8A
UniProt Synonym Gene Names
KIAA1437; LRRC8; SWELL1
UniProt Entry Name
LRC8A_HUMAN
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Product Notes
The LRRC8A lrrc8a (Catalog #AAA212530) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-LRRC8A Antibody, Affinity Purified reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LRRC8A can be used in a range of immunoassay formats including, but not limited to, IP (Immunoprecipitation), WB (Western Blot). Researchers should empirically determine the suitability of the LRRC8A lrrc8a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRRC8A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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