FCM/FACS (Flow Cytometry)
(LY75 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Rabbit anti-Human LY75 Polyclonal Antibody | anti-LY75 antibody
LY75 Antibody (Center)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LY75, Antibody; LY75 Antibody (Center); Lymphocyte antigen 75; Ly-75; C-type lectin domain family 13 member B; DEC-205; gp200-MR6; CD205; LY75; CLEC13B; anti-LY75 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LY75 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 646-675 amino acids from the Central region of human LY75.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
646-675
Sequence Length
232
Applicable Applications for anti-LY75 antibody
FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
FCM/FACS (Flow Cytometry)
(LY75 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
FCM/FACS (Flow Cytometry)
(LY75 Antibody (Center) flow cytometric analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(Formalin-fixed and paraffin-embedded human spleen tissue reacted with LY75 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of LY75 Antibody (Center) in HL-60 cell line lysates (35ug/lane). LY75 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of LY75 Antibody (Center) in HL-60 cell line lysates (35ug/lane). LY75 (arrow) was detected using the purified Pab.)
Related Product Information for anti-LY75 antibody
LY75 acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment. It causes reduced proliferation of B-lymphocytes.
Product Categories/Family for anti-LY75 antibody
References
Kato,M., et.al., Immunogenetics 47 (6), 442-450 (1998)
McKay,P.F., et.al., Eur. J. Immunol. 28 (12), 4071-4083 (1998)
McKay,P.F., et.al., Eur. J. Immunol. 28 (12), 4071-4083 (1998)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
198311
NCBI Official Full Name
LY75-CD302 fusion protein isoform 1
NCBI Official Synonym Full Names
LY75-CD302 readthrough
NCBI Official Symbol
LY75-CD302
NCBI Protein Information
LY75-CD302 fusion protein
UniProt Protein Name
Lymphocyte antigen 75
UniProt Gene Name
LY75
UniProt Synonym Gene Names
CD205; CLEC13B; Ly-75
UniProt Entry Name
LY75_HUMAN
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Product Notes
The LY75 ly75 (Catalog #AAA284510) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 646-675. The LY75 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LY75 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry), IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the LY75 ly75 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LY75, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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