WB (Western Blot)
(Western Blot of Lysozyme (Hen Egg White) antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Lysozyme (Hen Egg White) antibody at 1:1,000 overnight at 4 deg C. HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).)
Rabbit Lysozyme Polyclonal Antibody | anti-LYZ antibody
Lysozyme antibody
Gene Names
LYZ; LZM
Reactivity
Chicken lysozyme
Applications
Immunohistochemistry, Western Blot, Immunoprecipitation, ELISA
Purity
Lysozyme antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Synonyms
Lysozyme, Antibody; Lysozyme antibody; Polyclonal Lysozyme; Anti-Lysozyme; EC 3.2.1.17; Lyz; 1 4 beta n acetylmuramidase c; LZM; Lysozyme C precursor; lysosyme; Lysozyme C; anti-LYZ antibody
Host
Rabbit
Reactivity
Chicken lysozyme
Clonality
Polyclonal
Specificity
Chicken lysozyme
Purity/Purification
Lysozyme antibody was purified by delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Form/Format
Liquid in 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01% Sodium Azide
Sequence Length
148
Applicable Applications for anti-LYZ antibody
IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), ELISA
Biological Significance
Lysozyme is a relatively small (129 AA) secretory enzyme that catalyzes the hydrolysis of beta-1,4 glucosidic linkages between N-acetylmuramic acid (NAM) and N-acetylglucosamine (NAG) comprising the cell walls of bacteria and to a lesser degree chitin oligomers. Lysozyme is common in animals and plants. In birds, lysozyme is also an exceptionally abundant protein in egg whites. Its biological function in fowl eggs is unclear. Hen egg white lysozyme "c" is the most commonly studied form and source of the enzyme.
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
Lysozyme antibody was raised in rabbit using full length protein corresponding to amino acids 1-129 of Hen Egg White lysozyme as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage and at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
WB (Western Blot)
(Western Blot of Lysozyme (Hen Egg White) antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Lysozyme (Hen Egg White) antibody at 1:1,000 overnight at 4 deg C. HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).)
WB (Western Blot)
(Western Blot of Lysozyme (Hen Egg White) antibody. Lane 1: Lysozyme (Hen Egg White). Load: 50 ng per lane. Lysozyme (Hen Egg White) antibody at 1:1,000 overnight at 4 deg C. HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking buffer for 30 min at RT. Size: 4.9 kDa, 4.9 kDa for Lysozyme (Hen Egg White).)
WB (Western Blot)
(Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane. Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 deg C. Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated Goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: Blocking buffer overnight at 4 deg C. Size: 4.9kDa, 5kDa for Lysozyme..)
WB (Western Blot)
(Western Blot of Lysozyme antibody. (Left Blot-grey) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Load: 0.5 ug per lane. Biotin Conjugated Rabbit anti Lysozyme antibody at 1:5000 for overnight at 4 deg C. Dylight 488 conjugated Streptavidin at 1:5,000 with Atto 425 conjugated Goat anti rabbit secondary antibody at 1:10,000 for 1.5 hrs at RT. (Right Blot-red) Lane 1: purified Lysozyme reduced. Lane 2: purified Lysozyme non-reduced. Dylight 488 conjugated Streptavidin at 1:5,000 with Dylight 549 conjugated secondary antibody at 1:10,000 for 1.5 hrs at RT. Block: Blocking buffer overnight at 4 deg C. Size: 4.9kDa, 5kDa for Lysozyme..)
Related Product Information for anti-LYZ antibody
Rabbit polyclonal Lysozyme antibody
Product Categories/Family for anti-LYZ antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
16,537 Da
NCBI Official Full Name
lysozyme (EC 3.2.1.17)
NCBI Official Synonym Full Names
lysozyme
NCBI Official Symbol
LYZ
NCBI Official Synonym Symbols
LZM
NCBI Protein Information
lysozyme C
UniProt Protein Name
Lysozyme C
UniProt Gene Name
LYZ
UniProt Synonym Gene Names
LZM
UniProt Entry Name
LYSC_HUMAN
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Product Notes
The LYZ lyz (Catalog #AAA75005) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lysozyme antibody reacts with Chicken lysozyme and may cross-react with other species as described in the data sheet. AAA Biotech's Lysozyme can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), WB (Western Blot), IP (Immunoprecipitation), ELISA. Researchers should empirically determine the suitability of the LYZ lyz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lysozyme, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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