Rabbit anti-Human LZTFL1 Polyclonal Antibody | anti-LZTFL1 antibody

Anti-LZTFL1 Antibody, Rabbit Polyclonal

Cat. Num. AAA256268

• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry, Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: LZTFL1, Antibody; Anti-LZTFL1 Antibody, Rabbit Polyclonal; LZTFL1 Antibody, Rabbit PAb, Antigen Affinity Purified; Anti-LZTFL1 Antibody; anti-LZTFL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LZTFL1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-LZTFL1 antibody: IP (Immunoprecipitation), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: E Coli-derived Human LZTFL1 fragment
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with E Coli-derived Human LZTFL1 fragment, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(LZTFL1 was immunoprecipitated using:Lane A:0.5 mg Jurkat Whole Cell Lysate4 uL anti-LZTFL1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-LZTFL1 rabbit polyclonal antibody,at 1:100 dilutionSecondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 35 kDaObserved band size :34 kDa)

IHC (Immunohiostchemistry)

(Immunochemical staining of human LZTFL1 in human testis with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot)

(Anti-LZTFL1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:35 kDaObserved band size:35 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-LZTFL1 antibody

Leucine Zipper Transcription Factor-like 1 (LZTFL1) is located in the chromosome region 3p21.3 where allelic loss and genetic alterations occur early and frequently in lung cancers. LZTFL1 is downregulated in epithelial tumors, including lung cancer, and functions as a tumor suppressor in gastric cancers. LZTFL1 inhibits lung tumorigenesis, possibly by maintaining epithelial cell differentiation and/or inhibition of signalings that lead to EMT and suggest that reactivation of LZTFL1 expression in tumor cells may be a novel lung cancer therapeutic approach. LZTFL1 has been identified as a BBS protein (BBS17), because patients with mutations in this gene exhibit the common Bardet-Biedl syndrome (BBS) phenotypes. LZTFL1 is a cilium protein and regulates animal weight and photoreceptor connecting cilium function probably by controlling microtubule assembly and protein trafficking in cilia.

References

Wei Q, et al. (2016) Lztfl1 suppresses lung tumorigenesis by maintaining differentiation of lung epithelial cells. Oncogene 35 (20): 2655-2663.Li S, et al. (2019) Tumor suppressive functions of lztfl1 in hepatocellular carcinoma. Onco Targets Ther 12 5537-5544.Jiang J, et al. (2016) Depletion of bbs protein lztfl1 affects growth and causes retinal degeneration in mice. J Genet Genomics 43 (6): 381-391.