<
IHC (Immunohistochemistry)
(Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit M Sirt3 Polyclonal Antibody | anti-Sirt3 antibody
M Sirt3 Antibody (C-term)
Gene Names
Sirt3; Sir2l3; AI848213; 2310003L23Rik
Reactivity
Mouse, hamster
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
M Sirt3, Antibody; M Sirt3 Antibody (C-term); NAD-dependent protein deacetylase sirtuin-3; 351-; Regulatory protein SIR2 homolog 3; SIR2-like protein 3; mSIR2L3; Sirt3; Sir2l3; anti-Sirt3 antibody
Host
Rabbit
Reactivity
Mouse, hamster
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Sirt3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 304-334 amino acids from the C-terminal region of Mouse Sirt3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
304-334
Sequence Length
257
Applicable Applications for anti-Sirt3 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Function
NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, PDHA1, SOD2, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.
Cellular Location
Isoform L: Mitochondrion matrix.
Tissue Location
Strongly expressed in liver and kidney. Weakly expressed in lung.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemistry)
(Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemistry)
(Sirt3 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Sirt3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Anti-Mouse Sirt3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(Anti-Mouse Sirt3 Antibody (C-term) at 1:2000 dilution + mouse liver lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST)
WB (Western Blot)
(Anti-Sirt3 Antibody (C-term) at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Sirt3 Antibody (C-term) at 1:2000 dilution + CHO whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Sirt3 Antibody (C-term) at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(Anti-Sirt3 Antibody (C-term) at 1:64000 dilution + mouse liver lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-Sirt3 antibody
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
Product Categories/Family for anti-Sirt3 antibody
References
Hirschey, M.D., et al. Nature 464(7285):121-125(2010)
Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010)
Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)
Pillai, V.B., et al. J. Biol. Chem. 285(5):3133-3144(2010)
Kim, H.S., et al. Cancer Cell 17(1):41-52(2010)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
36615
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-3 isoform 1
NCBI Official Synonym Full Names
sirtuin 3
NCBI Official Symbol
Sirt3
NCBI Official Synonym Symbols
Sir2l3; AI848213; 2310003L23Rik
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-3
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-3
UniProt Gene Name
Sirt3
UniProt Synonym Gene Names
Sir2l3; mSIR2L3
UniProt Entry Name
SIR3_MOUSE
Customer Reviews
Loading reviews...
Share Your Experience
Similar Products
Product Notes
The Sirt3 sirt3 (Catalog #AAA288158) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 304-334. The M Sirt3 Antibody (C-term) reacts with Mouse, hamster and may cross-react with other species as described in the data sheet. AAA Biotech's M Sirt3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Sirt3 sirt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "M Sirt3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.