Application Data
(Anti-SIRT3 Picoband antibody, AAA46086-2.jpgAll lanes: Anti SIRT3 (AAA46086) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 44KDObserved bind size: 44KD)
Rabbit NAD-dependent protein deacetylase sirtuin-3, mitochondrial Polyclonal Antibody | anti-SIRT3 antibody
Anti-SIRT3 Antibody
Gene Names
SIRT3; SIR2L3
Reactivity
Human, Rat. No cross reactivity with other proteins.
Applications
Western Blot
Purity
Immunogen affinity purified.
Synonyms
NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Antibody; Anti-SIRT3 Antibody; hSIRT 3 antibody; hSIRT3 antibody; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody; NAD dependent deacetylase sirtuin 3 mitochondrial antibody; NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody; Regulatory protein SIR2 homolog 3 antibody; Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody; Sir 2 like 3 antibody; SIR 2 like protein 3 antibody; SIR 3 antibody; SIR2 L3 antibody; Sir2 like 3 antibody; SIR2 like protein 3 antibody; SIR2-like protein 3 antibody; SIR2L3 antibody; SIR3_HUMAN antibody; SIRT 3 antibody; SIRT3 antibody; Sirtuin 3 antibody; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody; Sirtuin type 3 antibody; Sirtuin3 antibody; sirtuin 3; anti-SIRT3 antibody
Host
Rabbit
Reactivity
Human, Rat. No cross reactivity with other proteins.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
257
Applicable Applications for anti-SIRT3 antibody
WB (Western Blot)
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen
E.coli-derived human SIRT3 recombinant protein (Position: P66-K399). Human SIRT3 shares 86% amino acid (aa) sequence identity with mouse SIRT3.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Application Data
(Anti-SIRT3 Picoband antibody, AAA46086-2.jpgAll lanes: Anti SIRT3 (AAA46086) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 44KDObserved bind size: 44KD)
Application Data
(Anti-SIRT3 Picoband antibody, AAA46086-2.jpgAll lanes: Anti SIRT3 (AAA46086) at 0.5ug/mlLane 1: Rat Spleen Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 44KDObserved bind size: 44KD)
Application Data
(Anti-SIRT3 Picoband antibody, AAA46086-1.jpgAll lanes: Anti SIRT3 (AAA46086) at 0.5ug/mlWB: Recombinant Human SIRT3 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Application Data
(Anti-SIRT3 Picoband antibody, AAA46086-1.jpgAll lanes: Anti SIRT3 (AAA46086) at 0.5ug/mlWB: Recombinant Human SIRT3 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)
Related Product Information for anti-SIRT3 antibody
Description: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-3, mitochondrial(SIRT3) detection. Tested with WB in Human;Rat.
Background: NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. It is mapped to 11p15.5. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of SIRT3 in cultured cells increases respiration and decreases the production of reactive oxygen species. SIRT3 can function as a mitochondrial deacetylase. In addition to it, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
Background: NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. It is mapped to 11p15.5. Endogenous SIRT3 is a soluble protein located in the mitochondrial matrix. Overexpression of SIRT3 in cultured cells increases respiration and decreases the production of reactive oxygen species. SIRT3 can function as a mitochondrial deacetylase. In addition to it, human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
28,567 Da
NCBI Official Full Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial isoform b
NCBI Official Synonym Full Names
sirtuin 3
NCBI Official Symbol
SIRT3
NCBI Official Synonym Symbols
SIR2L3
NCBI Protein Information
NAD-dependent protein deacetylase sirtuin-3, mitochondrial; sir2-like 3; sirtuin type 3; SIR2-like protein 3; regulatory protein SIR2 homolog 3; NAD-dependent deacetylase sirtuin-3, mitochondrial; silent mating type information regulation 2, S.cerevisiae, homolog 3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase
UniProt Protein Name
NAD-dependent protein deacetylase sirtuin-3, mitochondrial
UniProt Gene Name
SIRT3
UniProt Synonym Gene Names
SIR2L3; hSIRT3
UniProt Entry Name
SIR3_HUMAN
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Product Notes
The SIRT3 sirt3 (Catalog #AAA46086) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SIRT3 Antibody reacts with Human, Rat. No cross reactivity with other proteins. and may cross-react with other species as described in the data sheet. AAA Biotech's NAD-dependent protein deacetylase sirtuin-3, mitochondrial can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot). Researchers should empirically determine the suitability of the SIRT3 sirt3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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