IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C20orf133) Polyclonal Antibody, Unconjugated (bs-18603R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Rabbit MACROD2/C20orf133 Polyclonal Antibody | anti-MACROD2/C20orf133 antibody
MACROD2/C20orf133 Polyclonal Antibody
Gene Names
MACROD2; C20orf133
Applications
ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry
Purity
Affinity purified by Protein A
Synonyms
MACROD2/C20orf133, Antibody; MACROD2/C20orf133 Polyclonal Antibody; ADP-ribose glycohydrolase MACROD2; MACRO domain-containing protein 2; O-acetyl-ADP-ribose deacetylase MACROD2; 3.5.1.-; [Protein ADP-ribosylaspartate] hydrolase MACROD2; 3.2.2.-; [Protein ADP-ribosylglutamate] hydrolase MACROD2; MACROD2; anti-MACROD2/C20orf133 antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purified by Protein A
Form/Format
Liquid; 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol
Applicable Applications for anti-MACROD2/C20orf133 antibody
ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry)
Immunogen
KLH conjugated synthetic peptide derived from human MACROD2/C20orf133
Epitope Specificity
1-100/448
Subcellular Location
Nucleus.
Similarity
Contains 1 Macro domain.
Function
Removes ADP-ribose from aspartate and glutamate residues in proteins bearing a single ADP-ribose moiety (PubMed: , PubMed: ). Inactive towards proteins bearing poly-ADP-ribose (PubMed: , PubMed: ). Deacetylates O-acetyl-ADP ribose, a signaling molecule generated by the deacetylation of acetylated lysine residues in histones and other proteins (PubMed: )
Preparation and Storage
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4°C.
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C20orf133) Polyclonal Antibody, Unconjugated (bs-18603R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
IHC (Immunohistochemistry)
(Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C20orf133) Polyclonal Antibody, Unconjugated (bs-18603R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)
Related Product Information for anti-MACROD2/C20orf133 antibody
MACROD2 (MACRO Domain Containing 2) is a Protein Coding gene. Diseases associated with MACROD2 include autism spectrum disorder and pervasive developmental disorder. GO annotations related to this gene include hydrolase activity, acting on glycosyl bonds and deacetylase activity. An important paralog of this gene is MACROD1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50 KDa
NCBI Official Full Name
O-acetyl-ADP-ribose deacetylase MACROD2 isoform 2
NCBI Official Synonym Full Names
MACRO domain containing 2
NCBI Official Symbol
MACROD2
NCBI Official Synonym Symbols
C20orf133
NCBI Protein Information
O-acetyl-ADP-ribose deacetylase MACROD2; MACRO domain-containing protein 2; [Protein ADP-ribosylglutamate] hydrolase
UniProt Protein Name
O-acetyl-ADP-ribose deacetylase MACROD2
UniProt Gene Name
MACROD2
UniProt Synonym Gene Names
C20orf133
UniProt Entry Name
MACD2_HUMAN
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Product Notes
The MACROD2/C20orf133 macrod2 (Catalog #AAA291803) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MACROD2/C20orf133 can be used in a range of immunoassay formats including, but not limited to, ELISA, ICC (Immunocytochemistry), IF (Immunofluorescence), IHC (Immunohistochemistry), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the MACROD2/C20orf133 macrod2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MACROD2/C20orf133, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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