IHC (Immunohiostchemistry)
(MAGEA12 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA12 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Human MAGEA12 Polyclonal Antibody | anti-MAGEA12 antibody
MAGEA12 Antibody (N-term)
Gene Names
MAGEA12; CT1.12; MAGE12
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MAGEA12, Antibody; MAGEA12 Antibody (N-term); Melanoma-associated antigen 12; Cancer/testis antigen 112; CT112; MAGE-12 antigen; MAGE12F antigen; MAGEA12; MAGE12; anti-MAGEA12 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MAGEA12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-108 amino acids from the N-terminal region of human MAGEA12.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
81-108
Sequence Length
314
Applicable Applications for anti-MAGEA12 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohiostchemistry)
(MAGEA12 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA12 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohiostchemistry)
(MAGEA12 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA12 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(MAGEA12 Antibody (S96) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEA12 antibody detected the MAGEA12 protein (arrow).)
WB (Western Blot)
(MAGEA12 Antibody (S96) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEA12 antibody detected the MAGEA12 protein (arrow).)
Related Product Information for anti-MAGEA12 antibody
MAGEA12 is a member of the melanoma antigen gene (MAGE) family. The proteins of this family are tumor-specific antigens that can be recognized by autologous cytolytic T lymphocytes. This gene is expressed in various tumors and tumor cell lines from different tissue origins, but not detected in normal tissues, except testis. The function of this gene is unknown. This and other MAGE genes form a gene cluster at chromosome Xq28 region.
Product Categories/Family for anti-MAGEA12 antibody
References
Mallon, A.M., et al., Genome Res. 10(6):758-775 (2000).
Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
Ding, M., et al., Biochem. Biophys. Res. Commun. 202(1):549-555 (1994).
De Smet, C., et al., Immunogenetics 39(2):121-129 (1994).
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).
Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
Ding, M., et al., Biochem. Biophys. Res. Commun. 202(1):549-555 (1994).
De Smet, C., et al., Immunogenetics 39(2):121-129 (1994).
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34836
NCBI Official Full Name
melanoma-associated antigen 12
NCBI Official Synonym Full Names
melanoma antigen family A12
NCBI Official Symbol
MAGEA12
NCBI Official Synonym Symbols
CT1.12; MAGE12
NCBI Protein Information
melanoma-associated antigen 12
UniProt Protein Name
Melanoma-associated antigen 12
UniProt Gene Name
MAGEA12
UniProt Synonym Gene Names
MAGE12; CT1.12
UniProt Entry Name
MAGAC_HUMAN
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Product Notes
The MAGEA12 magea12 (Catalog #AAA286327) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 81-108. The MAGEA12 Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEA12 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAGEA12 magea12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEA12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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