WB (Western Blot)
(Western blot analysis of different tissue lysates using rabbit pAb to microtubule associated protein 2A and 2B (MAP2A/B), AAA76742, dilution 1:50,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] caw cortex. Strong band at about 280kDa mark corresponds to the two major high molecular weight MAP2 isoforms referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected on the blot, but the MAP2C and MAP2D isoforms are not.)
Rabbit MAP2 (Microtubule Associated Protein 2) Polyclonal Antibody | anti-MAP2 antibody
MAP2 (Microtubule Associated Protein 2)
Gene Names
MAP2; MAP2A; MAP2B; MAP2C
Reactivity
Human, Mouse, Rat, Bovine
Applications
Immunofluorescence, Western Blot, Immunocytochemistry
Synonyms
MAP2 (Microtubule Associated Protein 2), Antibody; MAP2 (Microtubule Associated Protein 2); anti-MAP2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine
Clonality
Polyclonal
Isotype
Rabbit IgG
Form/Format
Liquid. Supplied as an aliquot of serum plus 5mM NaN3
Applicable Applications for anti-MAP2 antibody
IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry)
Immunogen
Recombinant human projection domain sequences, amino acids 377-1505
Preparation and Storage
Antibody can also be aliquotted and stored frozen at -20 degree C in a manual defrost freezer for sixmonths without detectable loss of activity. The antibody can be stored at 2 degree - 8 degree C for 1 month without detectable loss of activity. Avoid repeated freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of different tissue lysates using rabbit pAb to microtubule associated protein 2A and 2B (MAP2A/B), AAA76742, dilution 1:50,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] caw cortex. Strong band at about 280kDa mark corresponds to the two major high molecular weight MAP2 isoforms referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected on the blot, but the MAP2C and MAP2D isoforms are not.)
WB (Western Blot)
(Western blot analysis of different tissue lysates using rabbit pAb to microtubule associated protein 2A and 2B (MAP2A/B), AAA76742, dilution 1:50,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain, and [4] caw cortex. Strong band at about 280kDa mark corresponds to the two major high molecular weight MAP2 isoforms referred to as MAP2A and MAP2B. Smaller fragments of these isoforms are also detected on the blot, but the MAP2C and MAP2D isoforms are not.)
IF (Immunofluorescence)
(Immunofluorescent analysis of rat brain striatum stained with rabbit pAb to microtubule associated protein 2A and 2B isotypes (MAP2A/B), AAA76742, dilution 1:10,000 in red, and costained with chicken pAb to myelin basic protein (MBP) in green. The blue is Hoechst staining of nuclear DNA. The MAP2A/B antibody stains dendrites and perikarya of neurons, while the MBP antibody labels oligodendrocytes and myelin sheathes around axons.)
IF (Immunofluorescence)
(Immunofluorescent analysis of rat brain striatum stained with rabbit pAb to microtubule associated protein 2A and 2B isotypes (MAP2A/B), AAA76742, dilution 1:10,000 in red, and costained with chicken pAb to myelin basic protein (MBP) in green. The blue is Hoechst staining of nuclear DNA. The MAP2A/B antibody stains dendrites and perikarya of neurons, while the MBP antibody labels oligodendrocytes and myelin sheathes around axons.)
Related Product Information for anti-MAP2 antibody
Microtubules are 25nm diameter protein rods found in eukaryotic cells and are associated with a family of proteins called microtubule associated proteins (MAPs). MAPs play a crucial role in the regulation of microtubule dynamics and interactions in vivo. MAP2 isoforms are expressed only in neuronal perikarya and dendrites so appropriate antibodies are used to identify these structures in cell culture and sections.
It was made against a mixture of recombinant human projection domain sequences. It binds to the MAP2A and MAP2B isoforms but not the lower molecular weight MAP2C and MAP2D forms which lack the projection domain sequences. This antibody is of very high titre, working well at dilutions of 1:10,000.
It was made against a mixture of recombinant human projection domain sequences. It binds to the MAP2A and MAP2B isoforms but not the lower molecular weight MAP2C and MAP2D forms which lack the projection domain sequences. This antibody is of very high titre, working well at dilutions of 1:10,000.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
199,526 Da
NCBI Official Full Name
microtubule-associated protein 2 isoform 1
NCBI Official Synonym Full Names
microtubule-associated protein 2
NCBI Official Symbol
MAP2
NCBI Official Synonym Symbols
MAP2A; MAP2B; MAP2C
NCBI Protein Information
microtubule-associated protein 2; MAP-2
UniProt Protein Name
Microtubule-associated protein 2
UniProt Gene Name
MAP2
UniProt Synonym Gene Names
MAP-2
UniProt Entry Name
MTAP2_HUMAN
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Product Notes
The MAP2 map2 (Catalog #AAA76742) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP2 (Microtubule Associated Protein 2) reacts with Human, Mouse, Rat, Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's MAP2 (Microtubule Associated Protein 2) can be used in a range of immunoassay formats including, but not limited to, IF (Immunofluorescence), WB (Western Blot), ICC (Immunocytochemistry). Researchers should empirically determine the suitability of the MAP2 map2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP2 (Microtubule Associated Protein 2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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