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product-image-AAA288950_IHC10.jpg IHC (Immunohistochemistry) (Mouse Map4k3 Antibody (Center) (AAA288950)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map4k3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Rabbit Map4k3 Polyclonal Antibody | anti-Map4k3 antibody

Mouse Map4k3 Antibody (Center)

Gene Names
Map4k3; Glk; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1; 4833416M01Rik; 4833416M07Rik; 9530052P13Rik
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Map4k3, Antibody; Mouse Map4k3 Antibody (Center); Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3; Map4k3; anti-Map4k3 antibody
Ordering
Host
Rabbit
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Map4k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 501-529 amino acids from the Central region of mouse Map4k3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
501-529
Sequence Length
894
Applicable Applications for anti-Map4k3 antibody
IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

IHC (Immunohistochemistry)

(Mouse Map4k3 Antibody (Center) (AAA288950)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map4k3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

product-image-AAA288950_IHC10.jpg IHC (Immunohistochemistry) (Mouse Map4k3 Antibody (Center) (AAA288950)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map4k3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

FCM/FACS (Flow Cytometry)

(Mouse Map4k3 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

product-image-AAA288950_FCM11.jpg FCM/FACS (Flow Cytometry) (Mouse Map4k3 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot)

(Mouse Map4k3 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)

product-image-AAA288950_WB13.jpg WB (Western Blot) (Mouse Map4k3 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)

WB (Western Blot)

(Mouse Map4k3 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)

product-image-AAA288950_WB15.jpg WB (Western Blot) (Mouse Map4k3 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)
Related Product Information for anti-Map4k3 antibody
Map4k3 may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).
Product Categories/Family for anti-Map4k3 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
101119
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Symbol
Map4k3
NCBI Official Synonym Symbols
Glk; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1; 4833416M01Rik; 4833416M07Rik; 9530052P13Rik
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 3
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 3
UniProt Gene Name
Map4k3
UniProt Synonym Gene Names
GLK; MEK kinase kinase 3; MEKKK 3
UniProt Entry Name
M4K3_MOUSE

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Product Notes

The Map4k3 map4k3 (Catalog #AAA288950) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 501-529. The Mouse Map4k3 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Map4k3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the Map4k3 map4k3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Map4k3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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