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WB (Western Blot)
(MAP4K3 western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAP4K3 antibody detected the MAP4K3 protein (arrow).)
Mouse anti-Human MAP4K3 Monoclonal Antibody | anti-MAP4K3 antibody
MAP4K3 Antibody
Gene Names
MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Synonyms
MAP4K3, Antibody; MAP4K3 Antibody; Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3; MAP4K3; RAB8IPL1; anti-MAP4K3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM,k
Clone Number
219CT8.3.1
Specificity
This MAP4K3 monoclonal antibody is generated from mouse immunized with MAP4K3 recombinant protein.
Form/Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Length
873
Applicable Applications for anti-MAP4K3 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Recombinant Protein
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(MAP4K3 western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAP4K3 antibody detected the MAP4K3 protein (arrow).)
WB (Western Blot)
(MAP4K3 western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the MAP4K3 antibody detected the MAP4K3 protein (arrow).)
WB (Western Blot)
(Western blot analysis of lysates from HepG2, MCF-7 cell line (from left to right), using MAP4K3 Antibody. AAA283442 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from HepG2, MCF-7 cell line (from left to right), using MAP4K3 Antibody. AAA283442 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using MAP4K3 Antibody. AAA283442 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
IHC (Immunohiostchemistry)
(Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using MAP4K3 Antibody. AAA283442 was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
WB (Western Blot)
(Western blot analysis of lysate from MCF-7 cell line, using MAP4K3. AAA283442 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
WB (Western Blot)
(Western blot analysis of lysate from MCF-7 cell line, using MAP4K3. AAA283442 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-MAP4K3 antibody
This gene encodes a member of the Ste20 family of
serine/threonine protein kinases. The protein belongs to the
subfamily that consists of members, such as germinal center kinase
(GCK), that are characterized by an N-terminal catalytic domain and
C-terminal regulatory domain. The kinase activity of the encoded
protein can be stimulated by UV radiation and tumor necrosis
factor-alpha. The protein specifically activates the c-Jun
N-terminal kinase (JNK) signaling pathway. Evidence suggests that
it functions upstream of mitogen-activated protein kinase kinase
kinase 1 (MEKK1). This gene previously was referred to as
RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed
mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).
serine/threonine protein kinases. The protein belongs to the
subfamily that consists of members, such as germinal center kinase
(GCK), that are characterized by an N-terminal catalytic domain and
C-terminal regulatory domain. The kinase activity of the encoded
protein can be stimulated by UV radiation and tumor necrosis
factor-alpha. The protein specifically activates the c-Jun
N-terminal kinase (JNK) signaling pathway. Evidence suggests that
it functions upstream of mitogen-activated protein kinase kinase
kinase 1 (MEKK1). This gene previously was referred to as
RAB8-interacting protein-like 1 (RAB8IPL1), but it has been renamed
mitogen-activated protein kinase kinase kinase kinase 3 (MAP4K3).
Product Categories/Family for anti-MAP4K3 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Yan, L., et al. Mol. Cell 37(5):633-642(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
Ichikawa, S., et al. J. Bone Miner. Res. 25(8):1821-1829(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Yan, L., et al. Mol. Cell 37(5):633-642(2010)
Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
101316
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase kinase 3
NCBI Official Symbol
MAP4K3
NCBI Official Synonym Symbols
GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
NCBI Protein Information
mitogen-activated protein kinase kinase kinase kinase 3
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase kinase 3
UniProt Gene Name
MAP4K3
UniProt Synonym Gene Names
RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
UniProt Entry Name
M4K3_HUMAN
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Product Notes
The MAP4K3 map4k3 (Catalog #AAA283442) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MAP4K3 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAP4K3 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAP4K3 map4k3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP4K3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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