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WB (Western Blot)
(Western blot analysis of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.)
Rabbit anti-Human, mouse MAPK12 Polyclonal Antibody | anti-MAPK12 antibody
MAPK12 Antibody (Center)
Gene Names
MAPK12; ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
Reactivity
Human, mouse
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MAPK12, Antibody; MAPK12 Antibody (Center); Mitogen-activated protein kinase 12; MAP kinase 12; MAPK 12; Extracellular signal-regulated kinase 6; ERK-6; Mitogen-activated protein kinase p38 gamma; MAP kinase p38 gamma; Stress-activated protein kinase 3; MAPK12; ERK6; SAPK3; anti-MAPK12 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MAPK12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human MAPK12.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
339-367
Sequence Length
357
Applicable Applications for anti-MAPK12 antibody
IHC (Immunohistochemistry), ELISA, WB (Western Blot)
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
WB (Western Blot)
(Western blot analysis of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.)
WB (Western Blot)
(Western blot analysis of MAPK12 (arrow) using rabbit polyclonal hMAPK12-P354. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPK12 gene.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
IHC (Immunohistochemisry)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
WB (Western Blot)
(Western blot analysis of p38 gamma (MAPK12) Antibody (Center) in mouse lung tissue lysates (35ug/lane). MAPK12 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(Western blot analysis of p38 gamma (MAPK12) Antibody (Center) in mouse lung tissue lysates (35ug/lane). MAPK12 (arrow) was detected using the purified Pab.)
WB (Western Blot)
(All lanes : Anti-MAPK12 Antibody (P354) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
WB (Western Blot)
(All lanes : Anti-MAPK12 Antibody (P354) at 1:2000 dilutionLane 1: Hela whole cell lysatesLane 2: Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 42 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-MAPK12 antibody
Activation of members of the mitogen-activated protein kinase family is a major mechanism for transduction of extracellular signals. Stress-activated protein kinases are one subclass of MAP kinases. MAPK12 functions as a signal transducer during differentiation of myoblasts to myotubes.
Product Categories/Family for anti-MAPK12 antibody
References
Garcia-Lora, A., et al., Cancer Immunol. Immunother. 52(1):59-64 (2003).
Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
Court, N.W., et al., J. Mol. Cell. Cardiol. 34(4):413-426 (2002).
Wang, X., et al., Mol. Cell. Biol. 20(13):4543-4552 (2000).
Julien, C., et al., J. Biol. Chem. 278(43):42615-42624 (2003).
Robinson, M.J., et al., J. Biol. Chem. 277(7):5094-5100 (2002).
Court, N.W., et al., J. Mol. Cell. Cardiol. 34(4):413-426 (2002).
Wang, X., et al., Mol. Cell. Biol. 20(13):4543-4552 (2000).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41940
NCBI Official Full Name
mitogen-activated protein kinase 12 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase 12
NCBI Official Symbol
MAPK12
NCBI Official Synonym Symbols
ERK3; ERK6; ERK-6; SAPK3; PRKM12; SAPK-3; MAPK 12; P38GAMMA
NCBI Protein Information
mitogen-activated protein kinase 12
UniProt Protein Name
Mitogen-activated protein kinase 12
UniProt Gene Name
MAPK12
UniProt Synonym Gene Names
ERK6; SAPK3; MAP kinase 12; MAPK 12; ERK-6; MAP kinase p38 gamma
UniProt Entry Name
MK12_HUMAN
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Product Notes
The MAPK12 mapk12 (Catalog #AAA290036) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 339-367. The MAPK12 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's MAPK12 can be used in a range of immunoassay formats including, but not limited to, IHC (Immunohistochemistry), ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MAPK12 mapk12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAPK12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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