IHC (Immunohistochemisry)
(Mouse Mapk3 Antibody (C-term) (AAA284012)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Rabbit anti-Mouse Mapk3 Polyclonal Antibody | anti-Mapk3 antibody
Mouse Mapk3 Antibody (C-term)
Gene Names
Mapk3; p44; Erk1; Ert2; Mnk1; Erk-1; Esrk1; Prkm3; Mtap2k; p44erk1; p44mapk
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Mapk3, Antibody; Mouse Mapk3 Antibody (C-term); Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; MNK1; Microtubule-associated protein 2 kinase; p44-ERK1; Mapk3; Erk1; Prkm3; anti-Mapk3 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Mouse Mapk3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 353-380 amino acids from the C-terminal region of mouse Mapk3.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
353-380
Sequence Length
380
Applicable Applications for anti-Mapk3 antibody
WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry)
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
MOUSE
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
IHC (Immunohistochemisry)
(Mouse Mapk3 Antibody (C-term) (AAA284012)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
IHC (Immunohistochemisry)
(Mouse Mapk3 Antibody (C-term) (AAA284012)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
WB (Western Blot)
(Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody (C-term). AAA284012 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
WB (Western Blot)
(Western blot analysis of lysates from mouse BA/F3, C2C12, NIH/3T3 and rat C6 cell line (from left to right), using Mouse Mapk3 Antibody (C-term). AAA284012 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284012 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FCM/FACS (Flow Cytometry)
(Flow cytometric analysis of Hela cells using Mouse Mapk3 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). AAA284012 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Related Product Information for anti-Mapk3 antibody
Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2) and heat shock factor protein 4 (HSF4) (By similarity). Phosphorylates SPZ1.
Product Categories/Family for anti-Mapk3 antibody
References
Chen, W., et al. Biochem. Biophys. Res. Commun. 401(3):339-343(2010)
Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
Alter, B.J., et al. J. Neurosci. 30(34):11537-11547(2010)
Spruce, T., et al. Dev. Cell 19(2):207-219(2010)
Bremer, J., et al. PLoS ONE 5 (8), E12450 (2010) :
Chandrakesan, P., et al. J. Biol. Chem. 285(43):33485-33498(2010)
Alter, B.J., et al. J. Neurosci. 30(34):11537-11547(2010)
Spruce, T., et al. Dev. Cell 19(2):207-219(2010)
Bremer, J., et al. PLoS ONE 5 (8), E12450 (2010) :
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
43066
NCBI Official Full Name
mitogen-activated protein kinase 3
NCBI Official Synonym Full Names
mitogen-activated protein kinase 3
NCBI Official Symbol
Mapk3
NCBI Official Synonym Symbols
p44; Erk1; Ert2; Mnk1; Erk-1; Esrk1; Prkm3; Mtap2k; p44erk1; p44mapk
NCBI Protein Information
mitogen-activated protein kinase 3
UniProt Protein Name
Mitogen-activated protein kinase 3
UniProt Gene Name
Mapk3
UniProt Synonym Gene Names
Erk1; Prkm3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
UniProt Entry Name
MK03_MOUSE
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Product Notes
The Mapk3 mapk3 (Catalog #AAA284012) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 353-380. The Mouse Mapk3 Antibody (C-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mapk3 can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry), ELISA, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the Mapk3 mapk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mapk3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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