WB (Western Blot)
(Western blot analysis of MARCH1 using LOVO whole cell lysates)
Rabbit MARCH1 Polyclonal Antibody | anti-MARCH1 antibody
MARCH1 Antibody
Gene Names
MARCH1; RNF171; MARCH-I
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MARCH1, Antibody; MARCH1 Antibody; DKFZp564M1682; E3 ubiquitin protein ligase MARCH1; E3 ubiquitin-protein ligase MARCH1; FLJ20668; MARCH 1; MARCH-I; March1; MARH1_HUMAN; Membrane associated RING CH protein I; Membrane associated ring finger (C3HC4) 1; Membrane associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase; Membrane associated RING finger protein 1; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; RING finger protein 171; RNF171; anti-MARCH1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MARCH1 antibody detects endogenous levels of total 42430
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
289
Applicable Applications for anti-MARCH1 antibody
ELISA, WB (Western Blot)
Immunogen
A synthesized peptide derived from human MARCH1
Subcellular Location
Lysosome; Endosome; Golgi Apparatus; Plasma Membrane
Tissue Specificity
Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells.
Predicted Cross Reactivity
Rabbit, Chicken
Similarity
Rabbit (100%), Chicken (86%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
WB (Western Blot)
(Western blot analysis of MARCH1 using LOVO whole cell lysates)
WB (Western Blot)
(Western blot analysis of MARCH1 using LOVO whole cell lysates)
WB (Western Blot)
(Western blot analysis of extracts from rat muscle, mouse brain, using MARCH1 Antibody.)
WB (Western Blot)
(Western blot analysis of extracts from rat muscle, mouse brain, using MARCH1 Antibody.)
Related Product Information for anti-MARCH1 antibody
Function: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.
Post-translational Modifications: Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins (By similarity).
Similarity: The RING-CH-type zinc finger domain is required for E3 ligase activity.
Post-translational Modifications: Has a short half-life. Instability/short half-life permits rapid changes that allow efficient induction of antigen presentation once antigen presenting cells, APCs, receive maturation signals. Small changes in protein levels significantly alter the cell surface display of MHC class II proteins (By similarity).
Similarity: The RING-CH-type zinc finger domain is required for E3 ligase activity.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 32 kDa
Predicted: 33 kDa
Predicted: 33 kDa
NCBI Official Full Name
E3 ubiquitin-protein ligase MARCH1 isoform 1
NCBI Official Synonym Full Names
membrane associated ring-CH-type finger 1
NCBI Official Symbol
MARCH1
NCBI Official Synonym Symbols
RNF171; MARCH-I
NCBI Protein Information
E3 ubiquitin-protein ligase MARCH1
UniProt Protein Name
E3 ubiquitin-protein ligase MARCH1
UniProt Gene Name
MARCH1
UniProt Synonym Gene Names
RNF171; MARCH-I
Similar Products
Product Notes
The MARCH1 march1 (Catalog #AAA327971) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MARCH1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MARCH1 can be used in a range of immunoassay formats including, but not limited to, ELISA, WB (Western Blot). Researchers should empirically determine the suitability of the MARCH1 march1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MARCH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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