Rabbit anti-Mouse MARCH1 Polyclonal Antibody | anti-MARCH1 antibody

MARCH1 Antibody (Center)

Cat. Num. AAA287384

• Gene Names: MARCH1; RNF171; MARCH-I
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: MARCH1, Antibody; MARCH1 Antibody (Center); E3 ubiquitin-protein ligase MARCH1; 632-; Membrane-associated RING finger protein 1; Membrane-associated RING-CH protein I; MARCH-I; RING finger protein 171; MARCH1; RNF171; anti-MARCH1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This MARCH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-191 amino acids from the Central region of human MARCH1.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 163-191
• Sequence Length: 272

• Applicable Applications for anti-MARCH1 antibody: ELISA, WB (Western Blot)
• Function: E3 ubiquitin-protein ligase that mediates ubiquitination of TFRC, CD86, FAS and MHC class II proteins, such as HLA-DR alpha and beta, and promotes their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. By constitutively ubiquitinating MHC class II proteins in immature dendritic cells, down-regulates their cell surface localization thus sequestering them in the intracellular endosomal system.
• Cellular Location: Golgi apparatus, trans-Golgi network membrane {ECO:0000250|UniProtKB:Q6NZQ8}; Multi-pass membrane protein. Lysosome membrane; Multi- pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein
• Tissue Location: Expressed in antigen presenting cells, APCs, located in lymph nodes and spleen. Also expressed in lung. Expression is high in follicular B-cells, moderate in dendritic cells and low in splenic T-cells.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(MARCH1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the MARCH1 antibody detected the MARCH1 protein (arrow).)

Related Product Information for anti-MARCH1 antibody

MARCH1 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH proteins add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH1 downregulates the surface expression of major histocompatibility complex (MHC) class II molecules (see MIM 142880) and other glycoproteins by directing them to the late endosomal/lysosomal compartment (Bartee et al., 2004 [PubMed 14722266]; Thibodeau et al., 2008 [PubMed 18389477]; De Gassart et al., 2008 [PubMed 18305173]).

Product Categories/Family for anti-MARCH1 antibody

Cell Biology; Immunology

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Thibodeau, J., et al. Eur. J. Immunol. 38(5):1225-1230(2008)
De Gassart, A., et al. Proc. Natl. Acad. Sci. U.S.A. 105(9):3491-3496(2008)
Bartee, E., et al. J. Virol. 78(3):1109-1120(2004)

NCBI and Uniprot Product Information

• NCBI GI #: 261823980
• NCBI GeneID: 55016
• NCBI Accession #: NP_001159845.1; NP_060393.1
• NCBI GenBank Nucleotide #: NM_001166373.1
• UniProt Accession #: Q8TCQ1; Q9NWR0; D3DP29
• Molecular Weight: 32308
• NCBI Official Full Name: E3 ubiquitin-protein ligase MARCH1 isoform 1
• NCBI Official Synonym Full Names: membrane-associated ring finger (C3HC4) 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: MARCH1
• NCBI Official Synonym Symbols: RNF171; MARCH-I
• NCBI Protein Information: E3 ubiquitin-protein ligase MARCH1
• UniProt Protein Name: E3 ubiquitin-protein ligase MARCH1
• UniProt Gene Name: MARCH1
• UniProt Synonym Gene Names: RNF171; MARCH-I
• UniProt Entry Name: MARH1_HUMAN