Rabbit Mcl-1 Polyclonal Antibody | anti-Mcl-1 antibody

RABBIT ANTI HUMAN Mcl-1

Cat. Num. AAA49988

• Gene Names: MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• Applications: Western Blot
• Synonyms: Mcl-1, Antibody; RABBIT ANTI HUMAN Mcl-1; anti-Mcl-1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises an epitope within the central region (IN) of human Myeloid cell leukaemia sequence 1 (MCL-1), an anti-apoptotic member of the Bcl-2 family of proteins, first identified in differentiating ML-1 myeloid cells, and expressed in many different cell lines, tissues and a variety of tumours. ; ; The MCL-1 protein, localised to intracellular membranes and particularly mitochondrial membranes, has since been isolated in two distinct isoforms, known as MCL-1 long (MCL-1L) and MCL-1 short (MCL-1S). MCL-1S has been identified as a pro-apoptotic splice variant of MCL-1L, missing the transmembrane and BH1 and BH2 (Bcl-2 homology) domains. Interaction between these two antagonistic variants can act to regulate cell survival. ; ; This item recognises both the MCL-1L (38kDa) and MCL-1S (30kDa) isoforms of MCL-1.
• Form/Format: Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 271

• Applicable Applications for anti-Mcl-1 antibody: WB (Western Blot)
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: A 16 amino acid peptide located near the centre of human MCL-1.
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human MCL-1 (IN) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
• Target Species: Human
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Application Data

(Immunocytochemical staining of Raji cells with Rabbit anti Human Mcl-1 (AAA49988))

Application Data

(Western blot analysis of whole cell lysate from Raji, Burkitt's lymphoma derived B-lymphoblastoid cells probed with Rabbit anti Human Mcl-1 (AAA49988) at 0.5(A), 1(B) and 2(C) ug/ml)

NCBI and Uniprot Product Information

• NCBI GI #: 309747067
• NCBI GeneID: 4170
• NCBI Accession #: NP_001184249.1
• NCBI GenBank Nucleotide #: NM_001197320.1
• UniProt Accession #: Q07820; Q9HD91; Q9NRQ3; Q9NRQ4; Q9UHR7; Q9UHR8; Q9UHR9; Q9UNJ1; B2R6B2; D3DV03; D3DV04
• NCBI Official Full Name: induced myeloid leukemia cell differentiation protein Mcl-1 isoform 3
• NCBI Official Synonym Full Names: myeloid cell leukemia 1
• NCBI Official Symbol: MCL1
• NCBI Official Synonym Symbols: TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT
• NCBI Protein Information: induced myeloid leukemia cell differentiation protein Mcl-1; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related)
• UniProt Protein Name: Induced myeloid leukemia cell differentiation protein Mcl-1
• UniProt Gene Name: MCL1
• UniProt Synonym Gene Names: BCL2L3; Bcl2-L-3
• UniProt Entry Name: MCL1_HUMAN