Rabbit MCSF Receptor Polyclonal Antibody | anti-MCSF antibody

Phospho-MCSF Receptor (Tyr723) Antibody

Cat. Num. AAA329438

• Gene Names: CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (83%), Dog (100%)
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: MCSF Receptor, Antibody; Phospho-MCSF Receptor (Tyr723) Antibody; C FMS; CD 115; CD115; CD115 antigen; CFMS; Colony stimulating factor 1 receptor; Colony stimulating factor I receptor; CSF 1 R; CSF 1R; CSF-1 receptor; CSF-1-R; CSF1 R; CSF1R; CSF1R_HUMAN; CSFR; EC 2.7.10.1; FIM 2; FIM2; FMS; FMS proto oncogene; FMS protooncogene; HDLS; M-CSF Receptor; M-CSF-R; Macrophage colony stimulating factor 1 receptor; Macrophage colony stimulating factor I receptor; Macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v fms) oncogene homolog; MCSFR; Oncogen FMS; Proto-oncogene c-Fms; V-FMS McDonough feline sarcoma viral oncogen homolog, formerly; anti-MCSF antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (92%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (83%), Dog (100%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-MCSF Receptor (Tyr723) Antibody detects endogenous levels of MCSF Receptor only when phosphorylated at Tyr723.; Tissue Specificity: Expressed in bone marrow and in differentiated blood mononuclear cells.
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-MCSF antibody: ELISA (Peptide ELISA), IHC (Immunohistochemistry), WB (Western Blot)
• Immunogen: A synthesized peptide derived from human MCSF Receptor around the phosphorylation site of Tyr723.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Autophosphorylated in response to CSF1 or IL34 binding. Phosphorylation at Tyr-561 is important for normal down-regulation of signaling by ubiquitination, internalization and degradation. Phosphorylation at Tyr-561 and Tyr-809 is important for interaction with SRC family members, including FYN, YES1 and SRC, and for subsequent activation of these protein kinases. Phosphorylation at Tyr-699 and Tyr-923 is important for interaction with GRB2. Phosphorylation at Tyr-723 is important for interaction with PIK3R1. Phosphorylation at Tyr-708 is important for normal receptor degradation. Phosphorylation at Tyr-723 and Tyr-809 is important for interaction with PLCG2. Phosphorylation at Tyr-969 is important for interaction with CBL. Dephosphorylation by PTPN2 negatively regulates downstream signaling and macrophage differentiation.Ubiquitinated. Becomes rapidly polyubiquitinated after autophosphorylation, leading to its degradation.
• Subunit Structure: Interacts with INPPL1/SHIP2 and THOC5 (By similarity). Monomer. Homodimer. Interacts with CSF1 and IL34. Interaction with dimeric CSF1 or IL34 leads to receptor homodimerization. Interacts (tyrosine phosphorylated) with PLCG2 (via SH2 domain). Interacts (tyrosine phosphorylated) with PIK3R1 (via SH2 domain). Interacts (tyrosine phosphorylated) with FYN, YES1 and SRC (via SH2 domain). Interacts (tyrosine phosphorylated) with CBL, GRB2 and SLA2.
• Similarity: The juxtamembrane domain functions as autoinhibitory region. Phosphorylation of tyrosine residues in this region leads to a conformation change and activation of the kinase.The activation loop plays an important role in the regulation of kinase activity. Phosphorylation of tyrosine residues in this region leads to a conformation change and activation of the kinase.Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
• Subcellular Location: Cell membrane>Single-pass type I membrane protein.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

IHC (Immunohiostchemistry)

(Staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot)

(Western blot analysis of extracts from 293 LPS 100ng/ml 30', using Phospho-M-CSF Receptor (Tyr723) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

Related Product Information for anti-MCSF antibody

Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone and tooth development. Required for normal male and female fertility, and for normal development of milk ducts and acinar structures in the mammary gland during pregnancy. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration, and promotes cancer cell invasion. Activates several signaling pathways in response to ligand binding. Phosphorylates PIK3R1, PLCG2, GRB2, SLA2 and CBL. Activation of PLCG2 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate, that then lead to the activation of protein kinase C family members, especially PRKCD. Phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leads to activation of the AKT1 signaling pathway. Activated CSF1R also mediates activation of the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1, and of the SRC family kinases SRC, FYN and YES1. Activated CSF1R transmits signals both via proteins that directly interact with phosphorylated tyrosine residues in its intracellular domain, or via adapter proteins, such as GRB2. Promotes activation of STAT family members STAT3, STAT5A and/or STAT5B. Promotes tyrosine phosphorylation of SHC1 and INPP5D/SHIP-1. Receptor signaling is down-regulated by protein phosphatases, such as INPP5D/SHIP-1, that dephosphorylate the receptor and its downstream effectors, and by rapid internalization of the activated receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 27262659
• NCBI GeneID: 1436
• NCBI Accession #: NP_005202.2
• NCBI GenBank Nucleotide #: NM_005211.3
• UniProt Accession #: P07333; Q6LDW5; Q6LDY4; Q86VW7; D3DQG2
• Molecular Weight: 107,984 Da
• NCBI Official Full Name: macrophage colony-stimulating factor 1 receptor
• NCBI Official Synonym Full Names: colony stimulating factor 1 receptor
• NCBI Official Symbol: CSF1R
• NCBI Official Synonym Symbols: FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
• NCBI Protein Information: macrophage colony-stimulating factor 1 receptor; CD115 antigen; CSF-1 receptor; FMS proto-oncogene; proto-oncogene c-Fms; macrophage colony stimulating factor I receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog
• UniProt Protein Name: Macrophage colony-stimulating factor 1 receptor
• UniProt Gene Name: CSF1R
• UniProt Synonym Gene Names: FMS; CSF-1-R; CSF-1R; M-CSF-R
• UniProt Entry Name: CSF1R_HUMAN